Which applications is the Mouse Anti-ACTR2 Recombinant Antibody (CBMAB-A1008-YC) validated for?

This antibody is validated for use in Western blot (WB), Immunofluorescence (IF), and Immunohistochemistry (IHC). Its high specificity and sensitivity make it a reliable tool for detecting ACTR2 protein in various sample types. We also have data supporting its use in ELISA applications.

What is the recommended working dilution for Western blot and Immunofluorescence?

For Western blotting, we recommend a starting dilution of 1:1,000 to 1:5,000, but optimal dilution may vary depending on the sample type and detection system. For Immunofluorescence, a concentration of 2-10 µg/ml is typically effective. We advise performing a quick dilution series to find the optimal concentration for your specific experiment to achieve the best signal-to-noise ratio.

Mouse Anti-ACTR2 Recombinant Antibody (V2-179656) (CBMAB-A1008-YC)

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Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179656

Application

ELISA, IHC-P, WB

Immunogen

ACTR2 (AAH14546.1, 1 a.a. ~ 394 a.a) full-length recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Actinin Alpha 2

Introduction

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamel

Entrez Gene ID

10097

UniProt ID

P61160

Alternative Names

ARP2 Actin Related Protein 2 Homolog; Actin-Like Protein 2; ARP2; ARP2 (Actin-Related Protein 2, Yeast) Homolog; ARP2 Actin-Related Protein 2 Homolog (Yeast); Actin-Related Protein 2;

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Biological Process

Arp2/3 complex-mediated actin nucleation
Associative learning
Asymmetric cell division
Cellular response to interferon-gamma
Cellular response to trichostatin A
Cilium assembly
Cytosolic transport
Ephrin receptor signaling pathway
Establishment or maintenance of cell polarity
Fc-gamma receptor signaling pathway involved in phagocytosis
Meiotic chromosome movement towards spindle pole
Meiotic cytokinesis
Membrane organization
Neutrophil degranulation
Positive regulation of dendritic spine morphogenesis
Positive regulation of double-strand break repair via homologous recombination
Positive regulation of lamellipodium assembly
Positive regulation of transcription by RNA polymerase II
Response to ethanol
Response to immobilization stress
Spindle localization

Cellular Location

Nucleus; Cytoskeleton; Cell projection

Yun, J., Ren, J., Liu, Y., Dai, L., Song, L., Ma, X., ... & Song, Y. (2021). Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy. Diabetology & Metabolic Syndrome, 13(1), 1-12.

Siebert, A. E., Hobbs, L. M., Johnston, A. J., Mishack, L. M., Brake, M. A., Cleuren, A., ... & Westrick, R. J. (2017). A Missense Mutation in the Loss-of-Function Intolerant Actr2 Gene Suppresses Thrombosis Via Multiple Mechanisms. Blood, 130(Supplement 1), 1058-1058.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Rabbit Anti-ACTR2 Recombinant Antibody (V2-179657) (CAT#: CBMAB-A1009-YC)

Mouse Anti-ACTR2 Recombinant Antibody (V2-12516) (CAT#: CBMAB-1006-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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