Mouse Anti-ACTR2 Recombinant Antibody (V2-12516) (CBMAB-1006-CN)

Name: Mouse Anti-ACTR2 Recombinant Antibody (V2-12516)
SKU: CBMAB-1006-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ACTR2 Recombinant Antibody (FMS96) (CBMAB-1006-CN) in IF

BLaER1 cells infected with Mtb6020 msp12∷cerulean demonstrate peripheral localization of Arp2, compared to interior localization of Arp2 in cells infected with Mtb6020 hsp60p∷esxM/msp12∷cerulean. Insets display fluorescence intensity of Arp2 and actin along the green line, with interior localization in Mtb6020 hsp60p∷esxM/msp12∷cerulean infections. ...View More

Saelens, J. W., Sweeney, M. I., Viswanathan, G., Xet-Mull, A. M., Smith, K. L. J., Sisk, D. M., ... & Tobin, D. M. (2022). An ancestral mycobacterial effector promotes dissemination of infection. Cell, 185(24), 4507-4525.

Mouse Anti-ACTR2 Recombinant Antibody (FMS96) (CBMAB-1006-CN) in IF

mCherry-hGBP1 was expressed in HeLa hGBP1-KO cells infected with S. flexneri ΔipaH9.8 and stained for IcsA, N-WASP, Arp2, and F-actin at 2.5 hpi. Colocalization of hGBP1 and indicated proteins on bacteria was quantified. Significance was determined by unpaired t-tests, two-tailed. n.s., not significant; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. ...View More

Kutsch, M., Sistemich, L., Lesser, C. F., Goldberg, M. B., Herrmann, C., & Coers, J. (2020). Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions. The EMBO journal, 39(13), e104926.

Mouse Anti-ACTR2 Recombinant Antibody (FMS96) (CBMAB-1006-CN) in IHC

Tubulobulbar complex and residual body formation requires KATNAL2.
(a-e) Electron microscopy showing elongated spermatid morphology during spermiation in Katnal2WT/WT and Katnal2Y86C/Y86C mice. Detachment of the acrosome (red arrowheads) from the nucleus was frequently observed in Katnal2Y86C/Y86C (b) but not Katnal2WT/WT (a) spermatids. Retention of cytoplasm was frequently seen in Katnal2Y86C/Y86C spermatids that had failed spermiation (c). Within the cytoplasm of retained spermatids, disorganised outer dense fibres (red arrowheads) were frequently observed (c). Ectoplasmic specializations (black arrowheads) showed normal morphology in Katnal2Y86C/Y86C (d) and Katnal2WT/WT (a) spermatids, and were absent in Katnal2Y86C/Y86C retained spermatids (e) indicating they were disassembled normally prior to spermiation. Tubulobulbar complexes (asterisks) were present in Katnal2WT/WT (a) spermatids but were absent in Katnal2Y86C/Y86C spermatids (d). Scale bars in a-e = 1 μm. Immunostaining of seminiferous tubules for the ectoplasmic specialization marker espin confirmed ectoplasmic specialisations were disassembled normally in Katnal2WT/WT and Katnal2Y86C/Y86C spermatids (f-k). Ectoplasmic specializations were present in early step 16 (stage VII) Katnal2WT/WT (f) and Katnal2Y86C/Y86C spermatids (i), but were absent from late step 16 (stage VIII) Katnal2WT/WT (g) and Katnal2Y86C/Y86C spermatids (j) and from retained (stage IX) Katnal2Y86C/Y86C spermatids (k). Immunostaining of seminiferous tubules for the tubulobulbar complex markers dynamin-2 and ARP2 confirmed the absence of tubulobulbar complexes from Katnal2Y86C/Y86C (o-p) but not Katnal2WT/WT (l-m) spermatids. Residual body (arrowheads) formation in Katnal2WT/WT (n) versus Katnal2Y86C/Y86C (q) mice. Residual bodies were absent in Katnal2Y86C/Y86C (q) mice. Scale bars in f-q = 10 μm. ...View More

Dunleavy, J. E., Okuda, H., O'Connor, A. E., Merriner, D. J., O'Donnell, L., Jamsai, D., ... & O'Bryan, M. K. (2017). Katanin-like 2 (KATNAL2) functions in multiple aspects of haploid male germ cell development in the mouse. PLoS genetics, 13(11), e1007078.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-12516

Application

ELISA, IF, WB

Immunogen

Recombinant full length protein (Human)

Host Species

Mouse

Specificity

Human, Mouse, Rat, Dog

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

0.02% sodium azide

Concentration

2.4 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Actinin Alpha 2

Introduction

The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusionThis protein functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. It seems to contact the pointed end of the daughter actin filament.

Entrez Gene ID

Human	10097
Mouse	66713
Rat	289820
Dog	481396

UniProt ID

Human	P61160
Mouse	P61161
Rat	Q5M7U6
Dog	E2QXY7

Alternative Names

ARP2

Function

ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Biological Process

Arp2/3 complex-mediated actin nucleation
Associative learning
Asymmetric cell division
Cellular response to interferon-gamma
Cellular response to trichostatin A
Cilium assembly
Cytosolic transport
Ephrin receptor signaling pathway
Establishment or maintenance of cell polarity
Fc-gamma receptor signaling pathway involved in phagocytosis
Meiotic chromosome movement towards spindle pole
Meiotic cytokinesis
Membrane organization
Neutrophil degranulation
Positive regulation of dendritic spine morphogenesis
Positive regulation of double-strand break repair via homologous recombination
Positive regulation of lamellipodium assembly
Positive regulation of transcription by RNA polymerase II
Response to ethanol
Response to immobilization stress
Spindle localization

Cellular Location

Nucleus; Cytoskeleton; Cell projection

Yun, J., Ren, J., Liu, Y., Dai, L., Song, L., Ma, X., ... & Song, Y. (2021). Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy. Diabetology & Metabolic Syndrome, 13(1), 1-12.

Siebert, A. E., Hobbs, L. M., Johnston, A. J., Mishack, L. M., Brake, M. A., Cleuren, A., ... & Westrick, R. J. (2017). A Missense Mutation in the Loss-of-Function Intolerant Actr2 Gene Suppresses Thrombosis Via Multiple Mechanisms. Blood, 130(Supplement 1), 1058-1058.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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