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Mouse Anti-ADAM22 Recombinant Antibody (V2-179776) (CBMAB-A1138-YC)

Provided herein is a Mouse monoclonal antibody against Human ADAM Metallopeptidase Domain 22. The antibody can be used for immunoassay techniques, such as ELISA, IP, WB.
See all ADAM22 antibodies
Published Data
Mouse Anti-ADAM22 Recombinant Antibody (C-2) (CBMAB-A1138-YC) in IHC
Immunohistochemical ADAM22 staining in 6 ER-positive endocrine-treated primary breast tumours and matched metastatic tissue. ADAM22 expression was maintained or elevated on metastasis. Scale bar, 50 μm. ...View More
Charmsaz, S., Doherty, B., Cocchiglia, S., Varešlija, D., Marino, A., Cosgrove, N., ... & Young, L. S. (2020). ADAM22/LGI1 complex as a new actionable target for breast cancer brain metastasis. BMC medicine, 18, 1-14.
Mouse Anti-ADAM22 Recombinant Antibody (C-2) (CBMAB-A1138-YC) in FC
After serum starvation, cells were incubated with LPS (5 μg/ml) for 24 h. Cells expressing LGI3 and ADAM22 were analyzed by flow cytometry. ...View More
Lee, S. H., Kwon, N. S., Baek, K. J., Yun, H. Y., & Kim, D. S. (2020). LGI3 is secreted and binds to ADAM22 via TRIF-dependent NF-κB pathway in response to LPS in human keratinocytes. Cytokine, 126, 154872.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-179776
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 286-319 within an internal region of ADAM22 of human origin
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 26-528

Target

Full Name
ADAM Metallopeptidase Domain 22
Introduction
ADAM22 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involvi
Entrez Gene ID
53616
UniProt ID
Q9P0K1
Alternative Names
ADAM Metallopeptidase Domain 22; Metalloproteinase-Like, Disintegrin-Like, And Cysteine-Rich Protein 2; A Disintegrin And Metalloproteinase Domain 22; Metalloproteinase-Disintegrin ADAM22-3; ADAM 22; MDC2; Disintegrin And Metalloproteinase Domain-Containi
Function
Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
Biological Process
Adult locomotory behavior
Cell adhesion
Central nervous system development
Myelination in peripheral nervous system
Negative regulation of cell adhesion
Neurotransmitter receptor localization to postsynaptic specialization membrane
Cellular Location
Cell membrane; Axon
Involvement in disease
Developmental and epileptic encephalopathy 61 (DEE61): A form of epileptic encephalopathy, a heterogeneous group of severe childhood onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE61 is an autosomal recessive condition characterized by onset of seizures in infancy.
Topology
Extracellular: 223-736 aa
Helical: 737-757 aa
Cytoplasmic: 758-906 aa
PTM
The precursor is cleaved by a furin endopeptidase.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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