What cell population is definitively labeled by this antibody, and what species reactivity is guaranteed for the F4/80 antigen?

This antibody specifically targets the ADGRE1 (F4/80) antigen, a canonical marker for tissue-resident macrophages in rodents. The reactivity is guaranteed for Mouse and often cross-reacts with Rat ADGRE1 (though confirmation should always be made on the batch-specific COA). It is crucial to note that this marker is not a reliable marker for human macrophages.

What quality control metrics are used to ensure lot-to-lot consistency, which is vital when quantifying immune cell populations by Flow Cytometry?

For FCM applications, consistency is assured by: 1) using a verified recombinant sequence (no hybridoma drift); 2) side-by-side validation of new lots against previous lots on positive cell controls (e.g., mouse spleen or liver macrophages); and 3) high purity (>95%) to ensure consistent Stain Index and minimal non-specific Fc receptor binding.

Rat Anti-ADGRE1 Recombinant Antibody (V2-160157) (CBMAB-F0005-CQ)

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Basic Information

Host Animal

Rat

Clone

V2-160157

Application

WB, IHC-F, IHC, IHC-P

Immunogen

Cultured macrophages.The F4/80 antigen is a 125 kD extracellular membrane protein sensitive to 2-Mercaptoethanol.

Host Species

Rat

Specificity

Human, Mouse

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-F	1:1,000
IHC-P	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.2, 1% BSA

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adhesion G Protein-Coupled Receptor E1

Introduction

This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

Human	2015
Mouse	13733

UniProt ID

Human	Q14246
Monse	Q61549

Alternative Names

Adhesion G Protein-Coupled Receptor E1; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 1; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1; EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 1; EGF-Like Module Receptor 1; EMR1 Hormone Receptor; TM7LN3; EMR1;

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Biological Process

Adaptive immune response
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell adhesion
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway

Cellular Location

Cell membrane

Topology

Extracellular: 21-599 aa
Helical: 600-627 aa
Cytoplasmic: 628-634 aa
Helical: 635-656 aa
Extracellular: 657-666 aa
Helical: 667-690 aa
Cytoplasmic: 691-709 aa
Helical: 710-731 aa
Extracellular: 732-747 aa
Helical: 748-776 aa
Cytoplasmic: 777-794 aa
Helical: 795-814 aa
Extracellular: 815-829 aa
Helical: 830-852 aa
Cytoplasmic: 853-886 aa

Waddell, L. A., Lefevre, L., Bush, S. J., Raper, A., Young, R., Lisowski, Z. M., ... & Hume, D. A. (2018). ADGRE1 (EMR1, F4/80) is a rapidly-evolving gene expressed in mammalian monocyte-macrophages. Frontiers in immunology, 9, 2246.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Rabbit Anti-ADGRE1 Recombinant Antibody (V2-160161) (CAT#: CBMAB-F0009-CQ)

Hamster Anti-ADGRE1 Recombinant Antibody (V2-160731) (CAT#: CBMAB-F0651-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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