Mouse Anti-ADH4 Recombinant Antibody (V2-179899) (CBMAB-A1283-YC)

Name: Mouse Anti-ADH4 Recombinant Antibody (V2-179899)
SKU: CBMAB-A1283-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADH4 Recombinant Antibody (F-6) (CBMAB-A1283-YC) in Immunoblot

Representative immunoblots of ADH1A, ADH1B, and ADH4 are shown with ERK2 as the loading control. Relative protein levels of all samples were determined by densitometry analysis normalized to ERK2 expression. Data are shown in box-whiskers graph (min to max value). (Normal, n = 8; steatosis, n = 4; NASH fatty, n= 11; NASH not fatty, n = 7). ...View More

Li, H., Toth, E., & Cherrington, N. J. (2018). Alcohol metabolism in the progression of human nonalcoholic steatohepatitis. Toxicological Sciences, 164(2), 428-438.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179899

Application

WB, IP, IF, ELISA

Immunogen

Raised against amino acids 36-75 mapping near the N-terminus of ADH4 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

alcohol dehydrogenase 4 (class II), pi polypeptide

Introduction

ADH4 is class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and l

Entrez Gene ID

127

UniProt ID

P08319

Alternative Names

Alcohol Dehydrogenase 4 (Class II), Pi Polypeptide; Alcohol Dehydrogenase Class II Pi Chain; EC 1.1.1.1; Epididymis Secretory Protein Li 4; Alcohol Dehydrogenase 4; Aldehyde Reductase; EC 1.1.1; HEL-S-4; ADH-2;

Function

Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Also catalyzes the reduction of benzoquinones.

Biological Process

Alcohol catabolic process
Alcohol metabolic process
Cellular aldehyde metabolic process
Ethanol oxidation
Fatty acid omega-oxidation
Formaldehyde catabolic process
Quinone metabolic process
Retinoid metabolic process
Retinol metabolic process

Cellular Location

Cytoplasm

Suo, C., Yang, Y., Yuan, Z., Zhang, T., Yang, X., Qing, T., ... & Ye, W. (2019). Alcohol intake interacts with functional genetic polymorphisms of aldehyde dehydrogenase (ALDH2) and alcohol dehydrogenase (ADH) to increase esophageal squamous cell cancer risk. Journal of Thoracic Oncology, 14(4), 712-725.

Guo, E., Wei, H., Liao, X., Xu, Y., Li, S., & Zeng, X. (2018). Prognostic value of alcohol dehydrogenase mRNA expression in gastric cancer. Oncology letters, 15(4), 5505-5516.

Edenberg, H. J., & McClintick, J. N. (2018). Alcohol dehydrogenases, aldehyde dehydrogenases, and alcohol use disorders: a critical review. Alcoholism: Clinical and Experimental Research, 42(12), 2281-2297.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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