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Mouse Anti-ADH5 Recombinant Antibody (V2-66965) (CBMAB-G1249-LY)

This product is antibody recognizes ADH5. The antibody CBLG1-325 immunoassay techniques such as: ELISA (EIA), WB.
See all ADH5 antibodies
Published Data
Mouse Anti-ADH5 Recombinant Antibody (CBLG1-325) (CBMAB-G1249-LY) in WB
Western blots of GSNOR along with the basal and phosphorylated forms of FAK1 and SRC, Mcl-1, and Bcl2 in extracts from WT, GSNOR-KO, and GSNOR-KO HeLa cells endogenously expressing FAK1 C459A or FAK1 C658S. LDH and vinculin were used as loading controls. Densitometry is shown on the right. ...View More
Cirotti, C., Rizza, S., Giglio, P., Poerio, N., Allega, M. F., Claps, G., ... & Filomeni, G. (2021). Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress. EMBO reports, 22(1), e50500.
Mouse Anti-ADH5 Recombinant Antibody (CBLG1-325) (CBMAB-G1249-LY) in IF
Representative fluorescence microscopy images of spheroids derived from WT, GSNOR-KO, and GSNOR-KO endogenously expressing FAK1 C459A or FAK1 C658S HeLa cells. Images represent maximum projections of ≥10 z stacks of 10 μm, and cells were stained as in (B). n refers to biologically independent experiments, while numbers in parentheses represent technical replicates. ...View More
Cirotti, C., Rizza, S., Giglio, P., Poerio, N., Allega, M. F., Claps, G., ... & Filomeni, G. (2021). Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress. EMBO reports, 22(1), e50500.
Mouse Anti-ADH5 Recombinant Antibody (CBLG1-325) (CBMAB-G1249-LY) in Immunoblot
Representative histograms and immunoblot images of alcohol dehydrogenase 5/S-nitrosoglutathione reductase (ADH5/GSNOR) and thioredoxin reductase 1 (TRXR1) (mean ± SD; * = significant difference, ANOVA and Tukey's test, n = 3, p-value < 0.05) in aged (A), young (Y), and centenarian (C) samples. ...View More
Capitanio, D., Barbacini, P., Arosio, B., Guerini, F. R., Torretta, E., Trecate, F., ... & Gelfi, C. (2020). Can serum nitrosoproteome predict longevity of aged women?. International Journal of Molecular Sciences, 21(23), 9009.
Mouse Anti-ADH5 Recombinant Antibody (CBLG1-325) (CBMAB-G1249-LY) in Immunoblot
Western blot analysis of GSNOR in HEK293 cells treated for 8 h with 100 μM H2O2 in the presence or absence of trigonelline (Trig). Densitometry of GSNOR immunoreactive bands is normalized to GADPH, selected as loading control, and expressed as arbitrary units. Values shown represent the means ± SD of n = 3 independent experiments. *P < 0.05; **P < 0.01. ...View More
Cirotti, C., Rizza, S., Giglio, P., Poerio, N., Allega, M. F., Claps, G., ... & Filomeni, G. (2021). Redox activation of ATM enhances GSNOR translation to sustain mitophagy and tolerance to oxidative stress. EMBO reports, 22(1), e50500.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-66965
Antibody Isotype
IgG1
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 322-347 of ADH5 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Alcohol Dehydrogenase 5 (Class III), Chi Polypeptide
Introduction
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
Entrez Gene ID
128
UniProt ID
P11766
Alternative Names
Alcohol Dehydrogenase 5 (Class III); Chi Polypeptide; Glutathione-Dependent Formaldehyde Dehydrogenase; S-(Hydroxymethyl)Glutathione Dehydrogenase; Alcohol Dehydrogenase Class Chi Chain; Alcohol Dehydrogenase Class-III; S-Nitrosoglutathione Reductase; Formaldehyde Dehydrogenase; EC 1.1.1.1; GSH-FDH; FALDH; ADHX; FDH;
Function
Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol.
Biological Process
Ethanol oxidation
Fatty acid omega-oxidation
Formaldehyde catabolic process
Peptidyl-cysteine S-nitrosylation
Positive regulation of blood pressure
Respiratory system process
Response to lipopolysaccharide
Response to nitrosative stress
Response to redox state
Retinoid metabolic process
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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