Mouse Anti-ADI1 Recombinant Antibody (V2-179917) (CBMAB-A1301-YC)

Provided herein is a Mouse monoclonal antibody against Human Acireductone Dioxygenase 1. The antibody can be used for immunoassay techniques, such as FC, IF, WB.
See all ADI1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179917

Antibody Isotype

IgG1

Application

IHC-F, IF, WB

Basic Information

Immunogen

Full length recombinant protein of human ADI1 (NP_060739) produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500
IF(ICC)	1:100
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Buffer

PBS, pH7.3, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acireductone Dioxygenase 1

Introduction

ADI1 is an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its loca

Entrez Gene ID

55256

UniProt ID

Q9BV57

Alternative Names

Acireductone Dioxygenase 1; Membrane-Type 1 Matrix Metalloproteinase Cytoplasmic Tail Binding Protein-1; Acireductone Dioxygenase (Fe(2+)-Requiring); Submergence-Induced Protein-Like Factor; Fe-ARD; MTCBP1; ARD; Membrane-Type 1 Matrix Metalloproteinase Cy

Function

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.

Biological Process

L-methionine salvage from methylthioadenosine
Methionine metabolic process

Cellular Location

Nucleus; Cell membrane; Cytoplasm. Localizes to the plasma membrane when complexed to MMP14.

References

Bae, D. H., Lane, D. J., Siafakas, A. R., Sutak, R., Paluncic, J., Huang, M. L., ... & Richardson, D. R. (2020). Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1866(10), 165844.

Chu, Y. D., Lai, H. Y., Pai, L. M., Huang, Y. H., Lin, Y. H., Liang, K. H., & Yeh, C. T. (2019). The methionine salvage pathway-involving ADI1 inhibits hepatoma growth by epigenetically altering genes expression via elevating S-adenosylmethionine. Cell death & disease, 10(3), 1-14.

Liu, X., & Pochapsky, T. C. (2019). Human Acireductone Dioxygenase (HsARD), Cancer and Human Health: Black Hat, White Hat or Gray?. Inorganics, 7(8), 101.

Deshpande, A. R., Pochapsky, T. C., Petsko, G. A., & Ringe, D. (2017). Dual chemistry catalyzed by human acireductone dioxygenase. Protein Engineering, Design and Selection, 30(3), 109-206.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ADI1 Recombinant Antibody (V2-634144)

Mouse Anti-ADI1 Recombinant Antibody (V2-179919)

Mouse Anti-ADI1 Recombinant Antibody (V2-179918)

Mouse Anti-ADI1 Recombinant Antibody (V2-179920)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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