Rabbit Anti-ADRA1B Recombinant Antibody (V2-12533) (CBMAB-1023-CN)

Name: Rabbit Anti-ADRA1B Recombinant Antibody (V2-12533)
SKU: CBMAB-1023-CN
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in WB

The protein expressions of α1b-AR on artificially induced decidual tissue of mice between the NE treatment group and the control group were detected by Western blot. ...View More

Wang, J., Tang, Y., Wang, S., Cui, L., Li, D., & Du, M. (2021). Norepinephrine exposure restrains endometrial decidualization during early pregnancy. Journal of Endocrinology, 248(3), 277-288.

Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in IHC

Immunohistochemical analysis of α1b-AR protein expression on artificially induced decidual tissue in mice. ...View More

Wang, J., Tang, Y., Wang, S., Cui, L., Li, D., & Du, M. (2021). Norepinephrine exposure restrains endometrial decidualization during early pregnancy. Journal of Endocrinology, 248(3), 277-288.

Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in PLA

Representative PLA images for the detection of receptor-receptor interactions. Images show merged PLA/4',6-diamidino-2-phenylindole dihydrochloride (DAPI) signals. Ctrl: Omission of one primary antibody. Scale bar = 10 μm. ...View More

Albee, L. J., Gao, X., & Majetschak, M. (2021). Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. Plos one, 16(6), e0253821.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-12533

Application

IHC-F, IF, WB

Immunogen

Synthetic peptide corresponding to Human alpha 1b Adrenergic Receptor aa. 150-250.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	1:500
WB	1:1,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 40% Glycerol, 0.05% BSA

Preservative

0.1% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adrenoceptor Alpha 1B

Introduction

Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.

Entrez Gene ID

Human	147
Mouse	11548
Rat	24173

UniProt ID

Human	P35368
Mouse	P97717
Rat	P15823

Alternative Names

ADRA1; ALPHA1BAR

Function

This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.

Biological Process

Adenylate cyclase-activating adrenergic receptor signaling pathway
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Cell-cell signaling
G protein-coupled receptor signaling pathway
Intracellular signal transduction
Neuron-glial cell signaling
Phospholipase C-activating G protein-coupled receptor signaling pathway
Positive regulation of cytosolic calcium ion concentration
Positive regulation of heart rate by epinephrine-norepinephrine
Positive regulation of MAPK cascade
Positive regulation of vasoconstriction
Regulation of cardiac muscle contraction

Cellular Location

Cytoplasm; Cell membrane; Nucleus membrane; Caveola. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.

Topology

Extracellular: 1-45 aa
Helical: 46-70 aa
Cytoplasmic: 71-83 aa
Helical: 84-105 aa
Extracellular: 106-115 aa
Helical: 116-141 aa
Cytoplasmic: 142-161 aa
Helical: 162-184 aa
Extracellular: 185-201 aa
Helical: 202-224 aa
Cytoplasmic: 225-295 aa
Helical: 296-319 aa
Extracellular: 320-326 aa
Helical: 327-351 aa
Cytoplasmic: 352-520 aa

Kurahashi, M., Kito, Y., Hara, M., Takeyama, H., Sanders, K. M., & Hashitani, H. (2020). Norepinephrine Has Dual Effects on Human Colonic Contractions Through Distinct Subtypes of Alpha 1 Adrenoceptors. Cellular and molecular gastroenterology and hepatology, 10(3), 658-671.

Wang, T., Qin, Y., Lai, H., Wei, W., Li, Z., Yang, Y., ... & Chen, J. (2019). The prognostic value of ADRA1 subfamily genes in gastric carcinoma. Oncology letters, 18(3), 3150-3158.

Adefurin, A., Ghimire, L. V., Kohli, U., Muszkat, M., Sofowora, G. G., Li, C., ... & Kurnik, D. (2017). Genetic variation in the alpha 1B-adrenergic receptor and vascular response. The pharmacogenomics journal, 17(4), 366-371.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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