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Rabbit Anti-ADRA1B Recombinant Antibody (V2-12533) (CBMAB-1023-CN)

This product is a rabbit antibody that recognizes ADRA1B of human. The antibody EPR10336 can be used for immunoassay techniques such as: FC, IF, WB.
See all ADRA1B antibodies
Published Data
Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in WB
The protein expressions of α1b-AR on artificially induced decidual tissue of mice between the NE treatment group and the control group were detected by Western blot. ...View More
Wang, J., Tang, Y., Wang, S., Cui, L., Li, D., & Du, M. (2021). Norepinephrine exposure restrains endometrial decidualization during early pregnancy. Journal of Endocrinology, 248(3), 277-288.
Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in IHC
Immunohistochemical analysis of α1b-AR protein expression on artificially induced decidual tissue in mice. ...View More
Wang, J., Tang, Y., Wang, S., Cui, L., Li, D., & Du, M. (2021). Norepinephrine exposure restrains endometrial decidualization during early pregnancy. Journal of Endocrinology, 248(3), 277-288.
Rabbit Anti-ADRA1B Recombinant Antibody (EPR10336) (CBMAB-1023-CN) in PLA
Representative PLA images for the detection of receptor-receptor interactions. Images show merged PLA/4',6-diamidino-2-phenylindole dihydrochloride (DAPI) signals. Ctrl: Omission of one primary antibody. Scale bar = 10 μm. ...View More
Albee, L. J., Gao, X., & Majetschak, M. (2021). Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. Plos one, 16(6), e0253821.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-12533
Antibody Isotype
IgG
Application
IHC-F, IF, WB

Basic Information

Immunogen
Synthetic peptide corresponding to Human alpha 1b Adrenergic Receptor aa. 150-250.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-P1:500
WB1:1,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 40% Glycerol, 0.05% BSA
Preservative
0.1% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adrenoceptor Alpha 1B
Introduction
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Entrez Gene ID
Human147
Mouse11548
Rat24173
UniProt ID
HumanP35368
MouseP97717
RatP15823
Alternative Names
ADRA1; ALPHA1BAR
Function
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine (PE)-stimulated ERK signaling in cardiac myocytes.
Biological Process
Adenylate cyclase-activating adrenergic receptor signaling pathway
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Cell-cell signaling
G protein-coupled receptor signaling pathway
Intracellular signal transduction
Neuron-glial cell signaling
Phospholipase C-activating G protein-coupled receptor signaling pathway
Positive regulation of cytosolic calcium ion concentration
Positive regulation of heart rate by epinephrine-norepinephrine
Positive regulation of MAPK cascade
Positive regulation of vasoconstriction
Regulation of cardiac muscle contraction
Cellular Location
Cytoplasm; Cell membrane; Nucleus membrane; Caveola. Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes.
Topology
Extracellular: 1-45 aa
Helical: 46-70 aa
Cytoplasmic: 71-83 aa
Helical: 84-105 aa
Extracellular: 106-115 aa
Helical: 116-141 aa
Cytoplasmic: 142-161 aa
Helical: 162-184 aa
Extracellular: 185-201 aa
Helical: 202-224 aa
Cytoplasmic: 225-295 aa
Helical: 296-319 aa
Extracellular: 320-326 aa
Helical: 327-351 aa
Cytoplasmic: 352-520 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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