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Mouse Anti-AFP Recombinant Antibody (V2-163277) (CBMAB-F3436-CQ)

This product is a mouse antibody that recognizes AFP. The antibody C3 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all AFP antibodies
Published Data
Mouse Anti-AFP Recombinant Antibody (C3) (CBMAB-F3436-CQ) in IF
Upper panel; coronal sections brain with teratoma stained with hematoxylin and eosin. Scale bar 100 µM. Lower panels; immunostaining: α-fetoprotein (AFP) for endoderm-derived tissue, α-smooth muscle actin (SMA) for mesoderm-derived tissue and Beta-III tubulin (βIII tub) for ectoderm-derived tissue. ...View More
Locatelli, M., Delhaes, F., Cherpin, O., Black, M. E., Carnesecchi, S., Preynat-Seauve, O., ... & Krause, K. H. (2022). Optimization of Thymidine Kinase-Based Safety Switch for Neural Cell Therapy. Cells, 11(3), 502.
Mouse Anti-AFP Recombinant Antibody (C3) (CBMAB-F3436-CQ) in WB
Western blots of GPC3 and AFP in DMSO or PRI-724-treated HepG2 and Huh6 cells. ...View More
Gulati, R., Hanlon, M. A., Lutz, M., Quitmeyer, T., Geller, J., Tiao, G., ... & Timchenko, N. (2022). Phosphorylation-Mediated Activation of β-Catenin-TCF4-CEGRs/ALCDs Pathway Is an Essential Event in Development of Aggressive Hepatoblastoma. Cancers, 14(24), 6062.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-163277
Antibody Isotype
IgG2a
Application
ELISA, IHC, WB

Basic Information

Immunogen
Amino acids 1-609 representing full length AFP of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)1:50-1:500
ELISA1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha Fetoprotein
Introduction
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Entrez Gene ID
Human174
Dog403551
UniProt ID
HumanP02771
DogQ8MJU5
Alternative Names
Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA;
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Biological Process
Cellular protein metabolic process
Post-translational protein modification
Cellular Location
Secreted
Involvement in disease
Alpha-fetoprotein deficiency (AFPD): A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development.
Alpha-fetoprotein, hereditary persistence (HPAFP): A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life.
PTM
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Zhu, A. X., Finn, R. S., Kang, Y. K., Yen, C. J., Galle, P. R., Llovet, J. M., ... & Kudo, M. (2021). Serum alpha-fetoprotein and clinical outcomes in patients with advanced hepatocellular carcinoma treated with ramucirumab. British journal of cancer, 124(8), 1388-1397.

Zhang, J., Chen, G., Zhang, P., Zhang, J., Li, X., Gan, D. N., ... & Ye, Y. A. (2020). The threshold of alpha-fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis. PLoS One, 15(2), e0228857.

Zheng, Y., Zhu, M., & Li, M. (2020). Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 1-8.

He, C., Peng, W., Liu, X., Li, C., Li, X., & Wen, T. F. (2019). Post-treatment alpha-fetoprotein response predicts prognosis of patients with hepatocellular carcinoma: a meta-analysis. Medicine, 98(31).

Galle, P. R., Foerster, F., Kudo, M., Chan, S. L., Llovet, J. M., Qin, S., ... & Zhu, A. X. (2019). Biology and significance of alpha‐fetoprotein in hepatocellular carcinoma. Liver international, 39(12), 2214-2229.

Mehta, N., Dodge, J. L., Roberts, J. P., Hirose, R., & Yao, F. Y. (2019). Alpha‐fetoprotein decrease from> 1,000 to< 500 ng/mL in patients with hepatocellular carcinoma leads to improved posttransplant outcomes. Hepatology, 69(3), 1193-1205.

Chan, M. Y., She, W. H., Dai, W. C., Tsang, S. H. Y., Chok, K. S. H., Chan, A. C. Y., ... & Cheung, T. T. (2019). Prognostic value of preoperative alpha-fetoprotein (AFP) level in patients receiving curative hepatectomy-an analysis of 1,182 patients in Hong Kong. Translational gastroenterology and hepatology, 4.

Tzartzeva, K., Obi, J., Rich, N. E., Parikh, N. D., Marrero, J. A., Yopp, A., ... & Singal, A. G. (2018). Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology, 154(6), 1706-1718.

Carr, B. I., Akkiz, H., Üsküdar, O., Yalçın, K., Guerra, V., Kuran, S., ... & Tokat, Y. (2018). HCC with low-and normal-serum alpha-fetoprotein levels. Clinical practice (London, England), 15(1), 453.

Li, G., Li, S., Wang, Z., Xue, Y., Dong, C., Zeng, J., ... & Zhou, Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, 37-44.

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For research use only. Not intended for any clinical use.

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