Cattle AFP ELISA Kit (V2LY-0626-LY2685)

Name: Cattle AFP ELISA Kit
SKU: V2LY-0626-LY2685
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.26 ng/mL

Detection Range

0.5-150 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Cattle

Assay Type

Sandwich

Reactivity

Cattle

Assay Time

1.5 h

Molecule Mass

68.6 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Alpha Fetoprotein

Alternative Names

Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA;

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Biological Process

Cellular protein metabolic process
Post-translational protein modification

Cellular Location

Secreted

Involvement in disease

Alpha-fetoprotein deficiency (AFPD): A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development.
Alpha-fetoprotein, hereditary persistence (HPAFP): A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life.

PTM

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Zhu, A. X., Finn, R. S., Kang, Y. K., Yen, C. J., Galle, P. R., Llovet, J. M., ... & Kudo, M. (2021). Serum alpha-fetoprotein and clinical outcomes in patients with advanced hepatocellular carcinoma treated with ramucirumab. British journal of cancer, 124(8), 1388-1397.

Zhang, J., Chen, G., Zhang, P., Zhang, J., Li, X., Gan, D. N., ... & Ye, Y. A. (2020). The threshold of alpha-fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis. PLoS One, 15(2), e0228857.

Zheng, Y., Zhu, M., & Li, M. (2020). Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 1-8.

He, C., Peng, W., Liu, X., Li, C., Li, X., & Wen, T. F. (2019). Post-treatment alpha-fetoprotein response predicts prognosis of patients with hepatocellular carcinoma: a meta-analysis. Medicine, 98(31).

Galle, P. R., Foerster, F., Kudo, M., Chan, S. L., Llovet, J. M., Qin, S., ... & Zhu, A. X. (2019). Biology and significance of alpha‐fetoprotein in hepatocellular carcinoma. Liver international, 39(12), 2214-2229.

Mehta, N., Dodge, J. L., Roberts, J. P., Hirose, R., & Yao, F. Y. (2019). Alpha‐fetoprotein decrease from> 1,000 to< 500 ng/mL in patients with hepatocellular carcinoma leads to improved posttransplant outcomes. Hepatology, 69(3), 1193-1205.

Chan, M. Y., She, W. H., Dai, W. C., Tsang, S. H. Y., Chok, K. S. H., Chan, A. C. Y., ... & Cheung, T. T. (2019). Prognostic value of preoperative alpha-fetoprotein (AFP) level in patients receiving curative hepatectomy-an analysis of 1,182 patients in Hong Kong. Translational gastroenterology and hepatology, 4.

Tzartzeva, K., Obi, J., Rich, N. E., Parikh, N. D., Marrero, J. A., Yopp, A., ... & Singal, A. G. (2018). Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology, 154(6), 1706-1718.

Carr, B. I., Akkiz, H., Üsküdar, O., Yalçın, K., Guerra, V., Kuran, S., ... & Tokat, Y. (2018). HCC with low-and normal-serum alpha-fetoprotein levels. Clinical practice (London, England), 15(1), 453.

Li, G., Li, S., Wang, Z., Xue, Y., Dong, C., Zeng, J., ... & Zhou, Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, 37-44.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human AFP ELISA Kit (CAT#: V2LY-0626-LY1827)

Mouse Afp ELISA Kit (CAT#: V2LY-0626-LY1947)

Horse AFP ELISA Kit (CAT#: V2LY-0626-LY489)

Pig AFP ELISA Kit (CAT#: V2LY-0626-LY927)

Human Recombinant AFP protein, hFc Tag (CAT#: V2LY-0526-LY2052)

Human Recombinant AFP protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2053)

Human Recombinant AFP protein, His Tag (CAT#: V2LY-0526-LY2054)

AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

Antibody Pairs

AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

Related Products

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AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

Mouse Anti-AFP Recombinant Antibody (CBT138) (CAT#: V2LY-0625-LY3771)

Mouse Anti-AFP Recombinant Antibody (V2-163277) (CAT#: CBMAB-F3436-CQ)

Mouse Anti-AFP Recombinant Antibody (CBT3) (CAT#: V2LY-0625-LY3879)

Mouse Anti-AFP Recombinant Antibody (CBT4562) (CAT#: V2LY-0625-LY1998)

Human Recombinant AFP protein, His Tag (CAT#: V2LY-0526-LY2054)

Human Recombinant AFP protein, hFc Tag (CAT#: V2LY-0526-LY2052)

Mouse Anti-AFP Recombinant Antibody (CBT2176) (CAT#: V2LY-0625-LY2374)

Mouse Anti-AFP Monoclonal Antibody (V2-632687) (CAT#: CBMAB-R0433-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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