Human Recombinant AFP protein, hFc Tag (V2LY-0526-LY2052)

Name: Human Recombinant AFP protein, hFc Tag
SKU: V2LY-0526-LY2052
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant AFP protein, hFc Tag consist of Amino Acid: 1-609 and predicts a molecular mass of 93.5 kDa.

Molecule Mass

93.5 kDa

Sequence

Amino Acid: 1-609

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Alpha Fetoprotein

Alternative Names

Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA;

Function

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Biological Process

Cellular protein metabolic process
Post-translational protein modification

Cellular Location

Secreted

Involvement in disease

Alpha-fetoprotein deficiency (AFPD): A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development.
Alpha-fetoprotein, hereditary persistence (HPAFP): A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life.

PTM

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Zhu, A. X., Finn, R. S., Kang, Y. K., Yen, C. J., Galle, P. R., Llovet, J. M., ... & Kudo, M. (2021). Serum alpha-fetoprotein and clinical outcomes in patients with advanced hepatocellular carcinoma treated with ramucirumab. British journal of cancer, 124(8), 1388-1397.

Zhang, J., Chen, G., Zhang, P., Zhang, J., Li, X., Gan, D. N., ... & Ye, Y. A. (2020). The threshold of alpha-fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis. PLoS One, 15(2), e0228857.

Zheng, Y., Zhu, M., & Li, M. (2020). Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 1-8.

He, C., Peng, W., Liu, X., Li, C., Li, X., & Wen, T. F. (2019). Post-treatment alpha-fetoprotein response predicts prognosis of patients with hepatocellular carcinoma: a meta-analysis. Medicine, 98(31).

Galle, P. R., Foerster, F., Kudo, M., Chan, S. L., Llovet, J. M., Qin, S., ... & Zhu, A. X. (2019). Biology and significance of alpha‐fetoprotein in hepatocellular carcinoma. Liver international, 39(12), 2214-2229.

Mehta, N., Dodge, J. L., Roberts, J. P., Hirose, R., & Yao, F. Y. (2019). Alpha‐fetoprotein decrease from> 1,000 to< 500 ng/mL in patients with hepatocellular carcinoma leads to improved posttransplant outcomes. Hepatology, 69(3), 1193-1205.

Chan, M. Y., She, W. H., Dai, W. C., Tsang, S. H. Y., Chok, K. S. H., Chan, A. C. Y., ... & Cheung, T. T. (2019). Prognostic value of preoperative alpha-fetoprotein (AFP) level in patients receiving curative hepatectomy-an analysis of 1,182 patients in Hong Kong. Translational gastroenterology and hepatology, 4.

Tzartzeva, K., Obi, J., Rich, N. E., Parikh, N. D., Marrero, J. A., Yopp, A., ... & Singal, A. G. (2018). Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology, 154(6), 1706-1718.

Carr, B. I., Akkiz, H., Üsküdar, O., Yalçın, K., Guerra, V., Kuran, S., ... & Tokat, Y. (2018). HCC with low-and normal-serum alpha-fetoprotein levels. Clinical practice (London, England), 15(1), 453.

Li, G., Li, S., Wang, Z., Xue, Y., Dong, C., Zeng, J., ... & Zhou, Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, 37-44.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AFP protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY2053)

Human Recombinant AFP protein, His Tag (CAT#: V2LY-0526-LY2054)

AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

Antibody Pairs

AFP Matched Antibody Pair (030) (CAT#: APMAB-030LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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