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Mouse Anti-AFP Monoclonal Antibody (V2-632688) (CBMAB-R0434-FY)

This product is mouse antibody that recognizes AFP. The antibody CBFYR0434 can be used for immunoassay techniques such as: ELISA.
See all AFP antibodies
Published Data
Mouse Anti-AFP Monoclonal Antibody (CBFYR0434) (CBMAB-R0434-FY) in FC
Detection of alpha-fetoprotein and glypican-3 antigen expression in Hepa1-6/GPC3 cells by flow cytometry. Flow cytometry data showed significantly higher alpha-fetoprotein expression in the cytoplasm (A) than in the membrane (B), compared with blank cell and IgG isotype controls. C: The positive shift of fluorescence distribution compared with isotype control illustrated higher membrane expression of the glypican-3 antigen. AFP: Alpha-fetoprotein; GPC3: Glypican-3. ...View More
Ma, X. H., Wang, S., Liu, S. Y., Chen, K., Wu, Z. Y., Li, D. F., ... & Zhao, X. M. (2019). Development and in vitro study of a bi-specific magnetic resonance imaging molecular probe for hepatocellular carcinoma. World Journal of Gastroenterology, 25(24), 3030.
Mouse Anti-AFP Monoclonal Antibody (CBFYR0434) (CBMAB-R0434-FY) in IHC
Unchanged tumor marker levels in liver, lung, brain, and skin of plasma-treated mice. Representative images of immunohistochemistry of different tumor markers (TM): AFP (I) in liver, β2M in brain (II), CEA in lung (III), as well as NSE staining in skin tissue (IV) after one year in positive control (+ve ctrl, left) and plasma-treated (right) animals. ...View More
Schmidt, A., von Woedtke, T., Stenzel, J., Lindner, T., Polei, S., Vollmar, B., & Bekeschus, S. (2017). One year follow-up risk assessment in SKH-1 mice and wounds treated with an argon plasma jet. International Journal of Molecular Sciences, 18(4), 868.
Mouse Anti-AFP Monoclonal Antibody (CBFYR0434) (CBMAB-R0434-FY) in ICC
Differentiation was performed using embryoid body formation, and the differentiated iPSCs (DP-A-iPS/hESF9 or DP-F-iPS/hESF9T) were fixed and reacted with antibodies. Shown were immunocytochemistry of Nestin, βIII-tubulin, α-smooth muscle actin (α-SMA), and α-fetoprotein (AFP). Binding of these antibodies was visualized with Alexa Fluor 488-conjugated secondary antibodies (green). Oct3/4 was also investigated. Binding of these antibodies was visualized with Alexa Fluor® 594-conjugated secondary antibodies (red). Nucleuses were stained with DAPI. (passage 25). Bar indicates 100 µm. ...View More
Yamasaki, S., Taguchi, Y., Shimamoto, A., Mukasa, H., Tahara, H., & Okamoto, T. (2014). Generation of human induced pluripotent stem (Ips) cells in serum-and feeder-free defined culture and TGF-β1 regulation of pluripotency. PLoS One, 9(1), e87151.

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
V2-632688
Antibody Isotype
IgG1, κ
Application
FC, IF, WB

Basic Information

Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)8-25 μg/ml
FC0.25 μg/10^6 cells
WB0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha Fetoprotein
Introduction
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Entrez Gene ID
174
UniProt ID
P02771
Alternative Names
Alpha Fetoprotein; Alpha-1-Fetoprotein; Alpha-Fetoglobulin; HPAFP; Alpha-Fetoprotein; AFPD; FETA
Function
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Biological Process
Cellular protein metabolic process
Post-translational protein modification
Cellular Location
Secreted
Involvement in disease
Alpha-fetoprotein deficiency (AFPD): A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development.
Alpha-fetoprotein, hereditary persistence (HPAFP): A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life.
PTM
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.

Zhu, A. X., Finn, R. S., Kang, Y. K., Yen, C. J., Galle, P. R., Llovet, J. M., ... & Kudo, M. (2021). Serum alpha-fetoprotein and clinical outcomes in patients with advanced hepatocellular carcinoma treated with ramucirumab. British journal of cancer, 124(8), 1388-1397.

Zhang, J., Chen, G., Zhang, P., Zhang, J., Li, X., Gan, D. N., ... & Ye, Y. A. (2020). The threshold of alpha-fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma: A systematic review and meta-analysis. PLoS One, 15(2), e0228857.

Zheng, Y., Zhu, M., & Li, M. (2020). Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 1-8.

He, C., Peng, W., Liu, X., Li, C., Li, X., & Wen, T. F. (2019). Post-treatment alpha-fetoprotein response predicts prognosis of patients with hepatocellular carcinoma: a meta-analysis. Medicine, 98(31).

Galle, P. R., Foerster, F., Kudo, M., Chan, S. L., Llovet, J. M., Qin, S., ... & Zhu, A. X. (2019). Biology and significance of alpha‐fetoprotein in hepatocellular carcinoma. Liver international, 39(12), 2214-2229.

Mehta, N., Dodge, J. L., Roberts, J. P., Hirose, R., & Yao, F. Y. (2019). Alpha‐fetoprotein decrease from> 1,000 to< 500 ng/mL in patients with hepatocellular carcinoma leads to improved posttransplant outcomes. Hepatology, 69(3), 1193-1205.

Chan, M. Y., She, W. H., Dai, W. C., Tsang, S. H. Y., Chok, K. S. H., Chan, A. C. Y., ... & Cheung, T. T. (2019). Prognostic value of preoperative alpha-fetoprotein (AFP) level in patients receiving curative hepatectomy-an analysis of 1,182 patients in Hong Kong. Translational gastroenterology and hepatology, 4.

Tzartzeva, K., Obi, J., Rich, N. E., Parikh, N. D., Marrero, J. A., Yopp, A., ... & Singal, A. G. (2018). Surveillance imaging and alpha fetoprotein for early detection of hepatocellular carcinoma in patients with cirrhosis: a meta-analysis. Gastroenterology, 154(6), 1706-1718.

Carr, B. I., Akkiz, H., Üsküdar, O., Yalçın, K., Guerra, V., Kuran, S., ... & Tokat, Y. (2018). HCC with low-and normal-serum alpha-fetoprotein levels. Clinical practice (London, England), 15(1), 453.

Li, G., Li, S., Wang, Z., Xue, Y., Dong, C., Zeng, J., ... & Zhou, Z. (2018). Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles. Analytical biochemistry, 547, 37-44.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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