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Rabbit Anti-AGR2 Recombinant Antibody (V2-180204) (CBMAB-A1628-YC)

Provided herein is a Rabbit monoclonal antibody against Human Anterior Gradient 2, Protein Disulphide Isomerase Family Member. The antibody can be used for immunoassay techniques, such as WB, IHC-P, IF, FC.
See all AGR2 antibodies
Published Data
Rabbit Anti-AGR2 Recombinant Antibody (D9V2F) (CBMAB-A1628-YC) in IF
(A-I) Krt5 (green) and Agr2 (red) staining and quantification 25 days after influenza. Agr2+ area per Krt5+ area was not significantly different between lung sections of (C) control (n=8) and Pou2f3-/- (n=8), (F) control (n=4) and Trpm5-/- (n=6) and (I) control (n=3) and Il4ra-/- (n=3). ...View More
Barr, J., Gentile, M. E., Lee, S., Kotas, M. E., de Mello Costa, M. F., Holcomb, N. P., ... & Vaughan, A. E. (2022). Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair. Elife, 11, e78074.
Rabbit Anti-AGR2 Recombinant Antibody (D9V2F) (CBMAB-A1628-YC) in IHC
Quantification of the percent of AGR2 positive cells in the IHC stains of Fast- and Slow-IGO transplants. Representative IHC images of IGO transplants of hF23 (left) and hM1A (right) at endpoint. Scale bars, 100 μm. Representative Data are means SEMs (n ≧ 3 fields of view). P value, unpaired Student's t test. ...View More
Miyabayashi, K., Baker, L. A., Deschênes, A., Traub, B., Caligiuri, G., Plenker, D., ... & Tuveson, D. A. (2020). Intraductal Transplantation Models of Human Pancreatic Ductal Adenocarcinoma Reveal Progressive Transition of Molecular SubtypesIntraductal Xenograft Models of Pancreatic Cancer. Cancer discovery, 10(10), 1566-1589.
Rabbit Anti-AGR2 Recombinant Antibody (D9V2F) (CBMAB-A1628-YC) in IHC
Expression of AGR2 in pituitary adenomas. (A) High expression of AGR2 (+++). (B) Intermediate expression of AGR2 (++). (C) Low expression of AGR2 (+). (D) AGR2-negative. Bar, 10 µm. AGR2, anterior gradient 2. ...View More
Tohti, M., Li, J., Ma, C., Li, W., Lu, Z., & Hu, Y. (2015). Expression of AGR2 in pituitary adenomas and its association with tumor aggressiveness. Oncology Letters, 10(5), 2878-2882.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-180204
Antibody Isotype
IgG
Application
WB, IHC-P, IF, IHC-F

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Ala29 of human AGR2 protein.
Host Species
Rabbit
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IHC-P1:800
IF(ICC)1:100
FC1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anterior Gradient 2, Protein Disulphide Isomerase Family Member
Introduction
AGR2 is a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thior
Entrez Gene ID
Human10551
Monkey709127
Frog549381
UniProt ID
HumanO95994
MonkeyA0A2K5V9R7
FrogQ90Y05
Alternative Names
Anterior Gradient 2, Protein Disulphide Isomerase Family Member; Protein Disulfide Isomerase Family A, Member 17; Secreted Cement Gland Protein XAG-2 Homolog; HAG-2; AG-2; HPC8; AG2; Anterior Gradient 2 Homolog (Xenopus Laevis); Anterior Gradient Protein
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
Biological Process
Digestive tract morphogenesis
Mucus secretion
Negative regulation of cell death
Positive regulation of cell-substrate adhesion
Positive regulation of developmental growth
Positive regulation of epidermal growth factor receptor signaling pathway
Positive regulation of gene expression
Positive regulation of IRE1-mediated unfolded protein response
Positive regulation of PERK-mediated unfolded protein response
Positive regulation of protein localization to plasma membrane
Cellular Location
Secreted; Endoplasmic reticulum

Wang, Y., Jia, M., Liang, C., Sheng, N., Wang, X., Wang, F., ... & Yuan, H. (2021). Anterior gradient 2 increases long-chain fatty acid uptake via stabilizing FABP1 and facilitates lipid accumulation. International journal of biological sciences, 17(3), 834.

Delom, F., Mohtar, M. A., Hupp, T., & Fessart, D. (2020). The anterior gradient-2 interactome. American Journal of Physiology-Cell Physiology, 318(1), C40-C47.

Moidu, N. A., Rahman, N. S. A., Syafruddin, S. E., Low, T. Y., & Mohtar, M. A. (2020). Secretion of pro-oncogenic AGR2 protein in cancer. Heliyon, 6(9), e05000.

Wang, D., Xu, Q., Yuan, Q., Jia, M., Niu, H., Liu, X., ... & Yuan, H. (2019). Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene, 38(18), 3458-3474.

Maurel, M., Obacz, J., Avril, T., Ding, Y. P., Papadodima, O., Treton, X., ... & Ogier‐Denis, E. (2019). Control of anterior GR adient 2 (AGR 2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO molecular medicine, 11(6), e10120.

Tiemann, K., Garri, C., Lee, S. B., Malihi, P. D., Park, M., Alvarez, R. M., ... & Kani, K. (2019). Loss of ER retention motif of AGR2 can impact mTORC signaling and promote cancer metastasis. Oncogene, 38(16), 3003-3018.

Zhang, Y., Xia, F., Zhang, F., Cui, Y., Wang, Q., Liu, H., & Wu, Y. (2019). miR-135b-5p enhances doxorubicin-sensitivity of breast cancer cells through targeting anterior gradient 2. Journal of Experimental & Clinical Cancer Research, 38(1), 1-13.

Li, J., Hu, J., Luo, Z., Zhou, C., Huang, L., Zhang, H., ... & Wang, Z. (2019). AGR2 is controlled by DNMT3a-centered signaling module and mediates tumor resistance to 5-Aza in colorectal cancer. Experimental cell research, 385(1), 111644.

Delom, F., Nazaraliyev, A., & Fessart, D. (2018). The role of protein disulphide isomerase AGR2 in the tumour niche. Biology of the Cell, 110(12), 271-282.

Zhu, Q., Mangukiya, H. B., Mashausi, D. S., Guo, H., Negi, H., Merugu, S. B., ... & Li, D. (2017). Anterior gradient 2 is induced in cutaneous wound and promotes wound healing through its adhesion domain. The FEBS journal, 284(17), 2856-2869.

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For research use only. Not intended for any clinical use.

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