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Mouse Anti-AGR2 Recombinant Antibody (V2-180192) (CBMAB-A1616-YC)

Provided herein is a Mouse monoclonal antibody against Human Anterior Gradient 2, Protein Disulphide Isomerase Family Member. The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AGR2 antibodies
Published Data
Mouse Anti-AGR2 Recombinant Antibody (1E5) (CBMAB-A1616-YC) in IHC
Micrographs of tissue sections from WT and Il18−/− distal colon stained to detect Muc2 and Apo-Muc2 (green) and Clca1, Zg16, and Agr2 (red); secreted Muc2 forming the IML is indicated (arrowheads). Error bars represent SEM of n = 4 (A) or n = 8 (D, F, G, and H) animals; ns, not significant; *, P < 0.05 significance determined by Kruskal-Wallis and uncorrected Dunn's multiple comparison. Scale bars, 100 µm. ...View More
Volk, J. K., Nyström, E. E., van der Post, S., Abad, B. M., Schroeder, B. O., Johansson, Å., ... & Birchenough, G. M. (2019). The Nlrp6 inflammasome is not required for baseline colonic inner mucus layer formation or function. Journal of Experimental Medicine, 216(11), 2602-2618.
Mouse Anti-AGR2 Recombinant Antibody (1E5) (CBMAB-A1616-YC) in WB
Western blot analysis of AGR2 expression in control and TMED2 overexpressing HEK293T cells upon autophagy inhibition with 20 and 10 μM chloroquine treatment. Actin (ACT) served as a loading control. ...View More
Maurel, M., Obacz, J., Avril, T., Ding, Y. P., Papadodima, O., Treton, X., ... & Ogier-Denis, E. (2019). Control of anterior GR adient 2 (AGR 2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO molecular medicine, 11(6), e10120.
Mouse Anti-AGR2 Recombinant Antibody (1E5) (CBMAB-A1616-YC) in Immunoblot
Spent culture media and lysates normalized to similar number of cells were immunoblotted using an anti-AGR2 antibody after reducing SDS-PAGE. Notice that bands blighted in the center have the highest concentration of AGR2. ...View More
Bergström, J. H., Berg, K. A., Rodríguez-Piñeiro, A. M., Stecher, B., Johansson, M. E., & Hansson, G. C. (2014). AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus. PLoS One, 9(8), e104186.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-180192
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Immunogen
Full-length recombinant protein corresponding to aa1-175 from AGR2 (AAH15503) with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS pH 7.4
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Anterior Gradient 2, Protein Disulphide Isomerase Family Member
Introduction
AGR2 is a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thior
Entrez Gene ID
10551
UniProt ID
O95994
Alternative Names
Anterior Gradient 2, Protein Disulphide Isomerase Family Member; Protein Disulfide Isomerase Family A, Member 17; Secreted Cement Gland Protein XAG-2 Homolog; HAG-2; AG-2; HPC8; AG2; Anterior Gradient 2 Homolog (Xenopus Laevis); Anterior Gradient Protein
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
Biological Process
Digestive tract morphogenesis
Mucus secretion
Negative regulation of cell death
Positive regulation of cell-substrate adhesion
Positive regulation of developmental growth
Positive regulation of epidermal growth factor receptor signaling pathway
Positive regulation of gene expression
Positive regulation of IRE1-mediated unfolded protein response
Positive regulation of PERK-mediated unfolded protein response
Positive regulation of protein localization to plasma membrane
Cellular Location
Secreted; Endoplasmic reticulum

Wang, Y., Jia, M., Liang, C., Sheng, N., Wang, X., Wang, F., ... & Yuan, H. (2021). Anterior gradient 2 increases long-chain fatty acid uptake via stabilizing FABP1 and facilitates lipid accumulation. International journal of biological sciences, 17(3), 834.

Delom, F., Mohtar, M. A., Hupp, T., & Fessart, D. (2020). The anterior gradient-2 interactome. American Journal of Physiology-Cell Physiology, 318(1), C40-C47.

Moidu, N. A., Rahman, N. S. A., Syafruddin, S. E., Low, T. Y., & Mohtar, M. A. (2020). Secretion of pro-oncogenic AGR2 protein in cancer. Heliyon, 6(9), e05000.

Wang, D., Xu, Q., Yuan, Q., Jia, M., Niu, H., Liu, X., ... & Yuan, H. (2019). Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene, 38(18), 3458-3474.

Maurel, M., Obacz, J., Avril, T., Ding, Y. P., Papadodima, O., Treton, X., ... & Ogier‐Denis, E. (2019). Control of anterior GR adient 2 (AGR 2) dimerization links endoplasmic reticulum proteostasis to inflammation. EMBO molecular medicine, 11(6), e10120.

Tiemann, K., Garri, C., Lee, S. B., Malihi, P. D., Park, M., Alvarez, R. M., ... & Kani, K. (2019). Loss of ER retention motif of AGR2 can impact mTORC signaling and promote cancer metastasis. Oncogene, 38(16), 3003-3018.

Zhang, Y., Xia, F., Zhang, F., Cui, Y., Wang, Q., Liu, H., & Wu, Y. (2019). miR-135b-5p enhances doxorubicin-sensitivity of breast cancer cells through targeting anterior gradient 2. Journal of Experimental & Clinical Cancer Research, 38(1), 1-13.

Li, J., Hu, J., Luo, Z., Zhou, C., Huang, L., Zhang, H., ... & Wang, Z. (2019). AGR2 is controlled by DNMT3a-centered signaling module and mediates tumor resistance to 5-Aza in colorectal cancer. Experimental cell research, 385(1), 111644.

Delom, F., Nazaraliyev, A., & Fessart, D. (2018). The role of protein disulphide isomerase AGR2 in the tumour niche. Biology of the Cell, 110(12), 271-282.

Zhu, Q., Mangukiya, H. B., Mashausi, D. S., Guo, H., Negi, H., Merugu, S. B., ... & Li, D. (2017). Anterior gradient 2 is induced in cutaneous wound and promotes wound healing through its adhesion domain. The FEBS journal, 284(17), 2856-2869.

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For research use only. Not intended for any clinical use.

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