Mouse Anti-AHSG Recombinant Antibody (V2-180308) (CBMAB-A1765-YC)

Name: Mouse Anti-AHSG Recombinant Antibody (V2-180308)
SKU: CBMAB-A1765-YC
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Published Data

Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunoblot

Validation immunoblot experiments and densitometric analyses showing protein expression in normal, early-stage, and late-stage myxomatous valves. ...View More

Lacerda, C. M., Disatian, S., & Orton, E. C. (2009). Differential protein expression between normal, early-stage, and late-stage myxomatous mitral valves from dogs. PROTEOMICS-Clinical Applications, 3(12), 1422-1429.

Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in IF

Confirmed the enhanced Fetuin-A in pal-induced insulin-resistant HepG2 cells by immunofluorescence. ...View More

Singh, P., Garg, R., Goand, U. K., Riyazuddin, M., Reza, M. I., Syed, A. A., ... & Gayen, J. R. (2020). Combination of Pancreastatin inhibitor PSTi8 with metformin inhibits Fetuin-A in type 2 diabetic mice. Heliyon, 6(10), e05133.

Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunoblot

Immunoblotting of Fetuin-A in the liver. ...View More

Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in Immunogold staining

Immunogold staining of mineral particles observed in human arteries of diabetic subjects and specimens prepared in vitro. Human artery tissues (A-O) and HS-particles prepared in vitro (P-T) and processed for thin-section TEM and immunogold labeling as described in the Methods. Gold-NP-coupled antibodies used for these experiments are indicated on top. An antibody that reacts against FenB, a protein from Bacillus subtilis, was used as a negative control. ApoA1, apolipoprotein-A1; FenB, fengycin B; HSA, human serum albumin; HSF, human serum fetuin-A; HS-NPs, human serum-nanoparticles. ...View More

Wu, C. Y., Martel, J., & Young, J. D. (2020). Ectopic calcification and formation of mineralo-organic particles in arteries of diabetic subjects. Scientific Reports, 10(1), 1-10.

Mouse Anti-AHSG Recombinant Antibody (CBYC-A451) (CBMAB-A1765-YC) in IF

Immunohistological staining of artery tissues from diabetic subjects showing colocalization of serum proteins and mineral deposits. Serial tissue sections were processed for albumin and fetuin-A immunofluorescence staining (A-D), von Kossa staining (E-H), and confocal fluorescence (I-L) as described in the Methods. White arrows indicate positive signals for immunofluorescence (albumin and/or fetuin-A) and von Kossa staining (calcification). White rectangles indicate the enlarged areas of the insets. TI, tunica intima; TM, tunica media. ...View More

Wu, C. Y., Martel, J., & Young, J. D. (2020). Ectopic calcification and formation of mineralo-organic particles in arteries of diabetic subjects. Scientific Reports, 10(1), 1-10.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-180308

Application

IHC, WB, ELISA, IP

Immunogen

Amino acids 68-367 of fetuin-A of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate).
IF(ICC)	1:50-1:500
ELISA	1:100-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alpha 2-HS Glycoprotein

Introduction

AHSG is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including

Entrez Gene ID

Human	197
Mouse	11625
Rat	25373

UniProt ID

Human	P02765
Mouse	P29699
Rat	P24090

Alternative Names

Alpha 2-HS Glycoprotein; Ba-Alpha-2-Glycoprotein; Alpha-2-Z-Globulin; Fetuin-A; FETUA; Alpha-2-HS-Glycoprotein;

Function

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

Biological Process

Acute-phase response Source: UniProtKB
Cellular protein metabolic process Source: Reactome
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of endopeptidase activity Source: GO_Central
Negative regulation of insulin receptor signaling pathway Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pinocytosis Source: UniProtKB
Platelet degranulation Source: Reactome
Positive regulation of phagocytosis Source: UniProtKB
Post-translational protein modification Source: Reactome
Regulation of bone mineralization Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Skeletal system development Source: UniProtKB

Cellular Location

Secreted

Involvement in disease

Alopecia-mental retardation syndrome 1 (APMR1): A rare autosomal recessive form of alopecia. APMR1 patients show loss of hair on the scalp, absence of eyebrows, eyelashes, axillary and pubic hair, and mild to severe mental retardation.

PTM

Phosphorylated by FAM20C in the extracellular medium.
O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).

Mancuso, E., Spiga, R., Rubino, M., Averta, C., Rotundo, S., Segura-Garcìa, C., ... & Andreozzi, F. (2021). Effects of Alpha-2-HS-glycoprotein on cognitive and emotional assessment in prediabetic and diabetic subjects. Journal of Affective Disorders, 282, 700-706.

Muzasti, R. A., Purwanto, B., & Sembiring, R. J. (2020). Is there an association between alpha 2-Heremans-Schmid glycoprotein (AHSG) gene Thr256Ser polymorphisms with aortic calcification in regular hemodialysis patients in Medan, Indonesia. Medicinski Glasnik, 17(2).

Roy, D., Fouzder, C., Mukhuty, A., Pal, S., Mondal, M. K., Kundu, R., & Chowdhury, P. (2019). Designed synthesis of dual emitting silicon quantum dot for cell imaging: direct labeling of alpha 2-HS-glycoprotein. Bioconjugate chemistry, 30(5), 1575-1583.

Lin, Y. H., Zhu, J., Meijer, S., Franc, V., & Heck, A. J. (2019). Glycoproteogenomics: A Frequent Gene Polymorphism Affects the Glycosylation Pattern of the Human Serum Fetuin/α-2-HS-Glycoprotein [S]. Molecular & Cellular Proteomics, 18(8), 1479-1490.

Heo, J. I., Yoon, D. W., Yu, J. H., Kim, N. H., Yoo, H. J., Seo, J. A., ... & Kim, N. H. (2018). Melatonin improves insulin resistance and hepatic steatosis through attenuation of alpha‐2‐HS‐glycoprotein. Journal of pineal research, 65(2), e12493.

Majek, P., Pecankova, K., Cermak, J., Gasova, Z., Prochazka, B., & Dyr, J. E. (2017). Alpha-2-HS-glycoprotein plasma level decrease correlates with age in patients with myelodysplastic syndromes. Cancer Biomarkers, 20(4), 637-639.

Kanno, T., Yasutake, K., Tanaka, K., Hadano, S., & Ikeda, J. E. (2017). A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection. PLoS One, 12(10), e0186227.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

AHSG Matched Antibody Pair (038) (CAT#: APMAB-038LY)

AHSG Matched Antibody Pair (039) (CAT#: APMAB-039LY)

AHSG Matched Antibody Pair (040) (CAT#: APMAB-040LY)

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Mouse Anti-AHSG Recombinant Antibody (V2-611056) (CAT#: CBMAB-A0209-LY)

Rabbit Anti-AHSG Recombinant Antibody (V2-160820) (CAT#: CBMAB-F0748-CQ)

AHSG Matched Antibody Pair (038) (CAT#: APMAB-038LY)

Mouse Anti-AHSG Recombinant Antibody (V2-180305) (CAT#: CBMAB-A1762-YC)

AHSG Matched Antibody Pair (039) (CAT#: APMAB-039LY)

Mouse Anti-AHSG Recombinant Antibody (V2-162541) (CAT#: CBMAB-F2651-CQ)

AHSG Matched Antibody Pair (040) (CAT#: APMAB-040LY)

Mouse Anti-AHSG Recombinant Antibody (V2-180304) (CAT#: CBMAB-A1761-YC)

Isotype control

For research use only. Not intended for any clinical use.

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