Rat Ahsg ELISA Kit (V2LY-0626-LY987)

Name: Rat Ahsg ELISA Kit
SKU: V2LY-0626-LY987
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.26 ng/mL

Detection Range

0.5-200 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

38.0 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Alpha 2-HS Glycoprotein

Function

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

Biological Process

Acute-phase response Source: UniProtKB
Cellular protein metabolic process Source: Reactome
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of endopeptidase activity Source: GO_Central
Negative regulation of insulin receptor signaling pathway Source: UniProtKB
Neutrophil degranulation Source: Reactome
Pinocytosis Source: UniProtKB
Platelet degranulation Source: Reactome
Positive regulation of phagocytosis Source: UniProtKB
Post-translational protein modification Source: Reactome
Regulation of bone mineralization Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Skeletal system development Source: UniProtKB

Cellular Location

Secreted

Involvement in disease

Alopecia-mental retardation syndrome 1 (APMR1): A rare autosomal recessive form of alopecia. APMR1 patients show loss of hair on the scalp, absence of eyebrows, eyelashes, axillary and pubic hair, and mild to severe mental retardation.

PTM

Phosphorylated by FAM20C in the extracellular medium.
O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).

Mancuso, E., Spiga, R., Rubino, M., Averta, C., Rotundo, S., Segura-Garcìa, C., ... & Andreozzi, F. (2021). Effects of Alpha-2-HS-glycoprotein on cognitive and emotional assessment in prediabetic and diabetic subjects. Journal of Affective Disorders, 282, 700-706.

Muzasti, R. A., Purwanto, B., & Sembiring, R. J. (2020). Is there an association between alpha 2-Heremans-Schmid glycoprotein (AHSG) gene Thr256Ser polymorphisms with aortic calcification in regular hemodialysis patients in Medan, Indonesia. Medicinski Glasnik, 17(2).

Roy, D., Fouzder, C., Mukhuty, A., Pal, S., Mondal, M. K., Kundu, R., & Chowdhury, P. (2019). Designed synthesis of dual emitting silicon quantum dot for cell imaging: direct labeling of alpha 2-HS-glycoprotein. Bioconjugate chemistry, 30(5), 1575-1583.

Lin, Y. H., Zhu, J., Meijer, S., Franc, V., & Heck, A. J. (2019). Glycoproteogenomics: A Frequent Gene Polymorphism Affects the Glycosylation Pattern of the Human Serum Fetuin/α-2-HS-Glycoprotein [S]. Molecular & Cellular Proteomics, 18(8), 1479-1490.

Heo, J. I., Yoon, D. W., Yu, J. H., Kim, N. H., Yoo, H. J., Seo, J. A., ... & Kim, N. H. (2018). Melatonin improves insulin resistance and hepatic steatosis through attenuation of alpha‐2‐HS‐glycoprotein. Journal of pineal research, 65(2), e12493.

Majek, P., Pecankova, K., Cermak, J., Gasova, Z., Prochazka, B., & Dyr, J. E. (2017). Alpha-2-HS-glycoprotein plasma level decrease correlates with age in patients with myelodysplastic syndromes. Cancer Biomarkers, 20(4), 637-639.

Kanno, T., Yasutake, K., Tanaka, K., Hadano, S., & Ikeda, J. E. (2017). A novel function of N-linked glycoproteins, alpha-2-HS-glycoprotein and hemopexin: Implications for small molecule compound-mediated neuroprotection. PLoS One, 12(10), e0186227.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant AHSG protein, His Tag (CAT#: V2LY-0526-LY8311)

Human Recombinant FETUA protein, His Tag (CAT#: V2LY-0526-LY4050)

Cattle AHSG ELISA Kit (CAT#: V2LY-0626-LY2477)

Human AHSG ELISA Kit (CAT#: V2LY-0626-LY3899)

AHSG Matched Antibody Pair (038) (CAT#: APMAB-038LY)

AHSG Matched Antibody Pair (039) (CAT#: APMAB-039LY)

AHSG Matched Antibody Pair (040) (CAT#: APMAB-040LY)

Antibody Pairs

AHSG Matched Antibody Pair (038) (CAT#: APMAB-038LY)

AHSG Matched Antibody Pair (039) (CAT#: APMAB-039LY)

AHSG Matched Antibody Pair (040) (CAT#: APMAB-040LY)

Related Products

AHSG Matched Antibody Pair (040) (CAT#: APMAB-040LY)

Human Recombinant FETUA protein, His Tag (CAT#: V2LY-0526-LY4050)

Rabbit Anti-AHSG Recombinant Antibody (V2-160820) (CAT#: CBMAB-F0748-CQ)

Mouse Anti-AHSG Recombinant Antibody (V2-180304) (CAT#: CBMAB-A1761-YC)

Rabbit Anti-AHSG Recombinant Antibody (V2-163221) (CAT#: CBMAB-F3379-CQ)

Mouse Recombinant AHSG protein, His Tag (CAT#: V2LY-0526-LY8311)

Mouse Anti-AHSG Recombinant Antibody (V2-611056) (CAT#: CBMAB-A0209-LY)

Cattle AHSG ELISA Kit (CAT#: V2LY-0626-LY2477)

Mouse Anti-AHSG Recombinant Antibody (V2-180308) (CAT#: CBMAB-A1765-YC)

Mouse Anti-AHSG Recombinant Antibody (V2-180305) (CAT#: CBMAB-A1762-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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