Mouse Anti-AIMP1 Recombinant Antibody (V2-43360) (CBMAB-E0251-FY)

Name: Mouse Anti-AIMP1 Recombinant Antibody (V2-43360)
SKU: CBMAB-E0251-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-43360

Application

WB, IHC, ICC

Immunogen

E.coli-derived recombinant human EMAP-II, Ser147-Lys312

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 147-12

More Infomation

Target

Full Name

Aminoacyl TRNA Synthetase Complex Interacting Multifunctional Protein 1

Introduction

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.

Entrez Gene ID

Human	9255
Mouse	13722
Rat	114632

UniProt ID

Human	Q12904
Mouse	P31230
Rat	Q4G079

Alternative Names

Aminoacyl TRNA Synthetase Complex Interacting Multifunctional Protein 1; Small Inducible Cytokine Subfamily E, Member 1 (Endothelial Monocyte-Activating); Multisynthase Complex Auxiliary Component P43; ARS-Interacting Multifunctional Protein 1; SCYE1; EMAP2; Aminoacyl TRNA Synthase Complex-Interacting Multifunctional Protein 1; Multisynthetase Complex Auxiliary Component P43

Function

Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (PubMed:10358004). Binds tRNA. Possesses inflammatory cytokine activity (PubMed:11306575). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (By similarity). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (By similarity). Promotes dermal fibroblast proliferation and wound repair (PubMed:16472771). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (By similarity). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations (PubMed:12237313). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed:11818442). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (PubMed:19362550).

Biological Process

Angiogenesis Source: UniProtKB-KW
Apoptotic process Source: UniProtKB-KW
Cell-cell signaling Source: HGNC-UCL
Defense response to virus Source: Ensembl
Inflammatory response Source: UniProtKB-KW
Leukocyte migration Source: HGNC-UCL
Negative regulation of endothelial cell proliferation Source: HGNC-UCL
Positive regulation of glucagon secretion Source: UniProtKB
tRNA aminoacylation for protein translation Source: Reactome

Cellular Location

Golgi apparatus; Secreted; Nucleus; Endoplasmic reticulum; Cytosol. Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Secreted in response to hypoxia (PubMed:10850427). Also secreted in response to both apoptotic and necrotic cell death.

Involvement in disease

Leukodystrophy, hypomyelinating, 3 (HLD3): A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system.

PTM

Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.

Lee, D. D., Hochstetler, A., Sah, E., Xu, H., Lowe, C. W., Santiaguel, S., ... & Schwarz, M. A. (2020). Influence of aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 on epithelial differentiation and organization during lung development. American Journal of Physiology-Lung Cellular and Molecular Physiology, 319(2), L369-L379.

Mun, C. H., Kim, J. O., Ahn, S. S., Yoon, T., Kim, S. J., Ko, E., ... & Park, S. G. (2019). Atializumab, a humanized anti-aminoacyl-tRNA synthetase-interacting multifunctional protein-1 (AIMP1) antibody significantly improves nephritis in (NZB/NZW) F1 mice. Biomaterials, 220, 119408.

Ahn, S. S., Kim, J. O., Yoon, T., Song, J. J., Park, Y. B., Lee, S. W., & Park, S. G. (2019). Serum aminoacyl-tRNA synthetase-interacting multifunctional protein-1 can predict severe antineutrophil cytoplasmic antibody-associated vasculitis: a pilot monocentric study. BioMed research international, 2019.

Accogli, A., Russell, L., Sébire, G., Rivière, J. B., St-Onge, J., Addour-Boudrahem, N., ... & Srour, M. (2019). Pathogenic variants in AIMP1 cause pontocerebellar hypoplasia. neurogenetics, 20(2), 103-108.

Khan, A., Bennett, J., Scantlebury, M. H., Wei, X. C., & Kerr, M. (2019). AIMP1 mutation long-term follow-up, with decreased brain N-acetylaspartic acid and secondary mitochondrial abnormalities. Child neurology open, 6, 2329048X19829520.

Kim, M. S., Song, J. H., Cohen, E. P., Cho, D., & Kim, T. S. (2017). Aminoacyl tRNA synthetase–interacting multifunctional protein 1 activates NK cells via macrophages in vitro and in vivo. The Journal of Immunology, 198(10), 4140-4147.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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