Mouse Anti-AK1 Recombinant Antibody (V2-180378) (CBMAB-A1850-YC)

Name: Mouse Anti-AK1 Recombinant Antibody (V2-180378)
SKU: CBMAB-A1850-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

V2-180378

Antibody Isotype

IgG2a, κ

Application

ELISA, IF, IP, WB

Basic Information

Immunogen

Amino acids 31-59 within an internal region of AK1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

adenylate kinase 1

Introduction

AK1 is an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in t

Entrez Gene ID

Human	203
Monkey	706338

UniProt ID

Human	P00568
Monkey	G8F558

Alternative Names

Adenylate Kinase 1; Adenylate Monophosphate Kinase; ATP-AMP Transphosphorylase 1; ATP:AMP Phosphotransferase; EC 2.7.4.3; Myokinase; Testis Secretory Sperm Binding Protein Li 58j;

Function

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.

Biological Process

ADP biosynthetic process Source: UniProtKB-UniRule
AMP metabolic process Source: UniProtKB-UniRule
ATP metabolic process Source: UniProtKB-UniRule
Nucleobase-containing small molecule interconversion Source: Reactome
Nucleoside diphosphate phosphorylation Source: UniProtKB
Nucleoside triphosphate biosynthetic process Source: UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Hemolytic anemia due to adenylate kinase deficiency (HAAKD): A disease characterized by hemolytic anemia and undetectable erythrocyte adenylate kinase activity.

More Infomation

References

Zervou, S., McAndrew, D. J., Whittington, H. J., Lake, H. A., Park, K. C., Cha, K. M., ... & Lygate, C. A. (2021). Subtle role for adenylate kinase 1 in maintaining normal basal contractile function and metabolism in the murine heart. Frontiers in physiology, 12, 454.

Tang, C. L., Zhang, R. H., Li, R., Li, X. R., Pan, Q., Li, L., & Xiao, J. L. (2021). EFFECT OF ADENYLATE KINASE 1 ON THE GROWTH AND DEVELOPMENT OF SCHISTOSOMA JAPONICUM SCHISTOSOMULUM. The Journal of Parasitology, 107(3), 472-480.

Xie, M., Zhang, G., Zhang, H., Chen, F., Chen, Y., Zhuang, Y., ... & Chen, Z. (2020). Adenylate kinase 1 deficiency disrupts mouse sperm motility under conditions of energy stress. Biology of Reproduction, 103(5), 1121-1131.

Gao, Y. R., Chen, L., Li, M. C., Li, Y. H., Lei, L., Wu, Q., ... & Li, L. (2020). Role of adenylate kinase 1 in the integument development of Schistosoma japonicum schistosomula. Acta tropica, 207, 105467.

Jan, Y. H., Lai, T. C., Yang, C. J., Huang, M. S., & Hsiao, M. (2019). A co-expressed gene status of adenylate kinase 1/4 reveals prognostic gene signature associated with prognosis and sensitivity to EGFR targeted therapy in lung adenocarcinoma. Scientific reports, 9(1), 1-11.

Gao, Y., Zhou, X., Wang, H., Liu, R., Ye, Q., Zhao, Q., ... & Dong, H. (2017). Immunization with recombinant schistosome adenylate kinase 1 partially protects mice against Schistosoma japonicum infection. Parasitology research, 116(6), 1665-1674.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

AK1 Matched Antibody Pair (044)

AK1 Matched Antibody Pair (045)

AK1 Matched Antibody Pair (046)

AK1 Matched Antibody Pair (047)

AK1 Matched Antibody Pair (048)

AK1 Matched Antibody Pair (049)

AK1 Matched Antibody Pair (050)

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AK1 Matched Antibody Pair (044)

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AK1 Matched Antibody Pair (050)

Mouse Anti-AK1 Recombinant Antibody (V2-180377)

Mouse Anti-AK1 Recombinant Antibody (V2-180376)

Mouse Anti-AK1 Recombinant Antibody (V2-180375)

AK1 Matched Antibody Pair (048)

AK1 Matched Antibody Pair (045)

Mouse Anti-AK1 Recombinant Antibody (V2-180380)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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