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Mouse Anti-AKAP4 Recombinant Antibody (V2-611074) (CBMAB-A0227-LY)

The product is antibody recognizes AKAP4. The antibody 4C10 immunoassay techniques such as: WB, ELISA.
See all AKAP4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-611074
Antibody Isotype
IgG2a, κ
Application
WB, ELISA, ICC/IF, IHC, IHC-P

Basic Information

Immunogen
AKAP4 (NP_003877, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
A kinase (PRKA) anchor protein 4
Introduction
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
Entrez Gene ID
8852
UniProt ID
Q5JQC9
Alternative Names
AKAP82; FSC1; HI; hAKAP82; p82
Function
Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Biological Process
Establishment of protein localization Source: Ensembl
Flagellated sperm motility Source: UniProtKB
Motile cilium assembly Source: Ensembl
Protein localization Source: GO_Central
Signal transduction Source: ProtInc
Single fertilization Source: ProtInc
Transmembrane receptor protein serine/threonine kinase signaling pathway Source: Ensembl
Cellular Location
Flagellum. Localizes to the principle piece of the sperm flagellum.

Choowong‐in, P., Sattayasai, J., Poodendaen, C., & Iamsaard, S. (2021). Decreased expression of AKAP4 and TyrPho proteins in testis, epididymis, and spermatozoa with low sexual performance of mice induced by modified CUMS. Andrologia, 53(3), e13977.

Blommaert, D., Sergeant, N., Delehedde, M., Jouy, N., Mitchell, V., Franck, T., ... & Serteyn, D. (2019). Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology, 131, 52-60.

Nixon, B., Bernstein, I. R., Cafe, S. L., Delehedde, M., Sergeant, N., Anderson, A. L., ... & Bromfield, E. G. (2019). A kinase anchor protein 4 is vulnerable to oxidative adduction in male germ cells. Frontiers in cell and developmental biology, 7, 319.

Li, Q., Tang, H., Hu, F., & Qin, C. (2018). Knockdown of A‐kinase anchor protein 4 inhibits hypoxia‐induced epithelial‐to‐mesenchymal transition via suppression of the Wnt/β‐catenin pathway in human gastric cancer cells. Journal of cellular biochemistry, 119(12), 10013-10020.

Jumeau, F., Sigala, J., Dossou‐Gbete, F., Frimat, K., Barbotin, A. L., Buee, L., ... & Mitchell, V. (2018). A‐kinase anchor protein 4 precursor (pro‐AKAP4) in human spermatozoa. Andrology, 6(6), 854-859.

Kumar, V., Jagadish, N., & Suri, A. (2017). Role of A-Kinase anchor protein (AKAP4) in growth and survival of ovarian cancer cells. Oncotarget, 8(32), 53124.

Han, J., Gao, W., Su, D., & Liu, Y. (2017). Silencing of A-kinase anchor protein 4 (AKAP4) inhibits proliferation and progression of thyroid cancer. Oncology research, 25(6), 873.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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