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Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292)


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Tested Data
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in ELISA
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in ELISA
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in WB
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in WB
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in WB
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in WB
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in FC
Mouse Anti-AKT1S1 Recombinant Antibody (CBT3742) (V2LY-0625-LY1292) in FC

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT3742
Antibody Isotype
IgG1
Application
FC

Basic Information

Immunogen
Purified recombinant fragment of human AKT1S1 (AA: 92-276) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
FC1:200-1:400
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
84335
UniProt ID
Q96B36
Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Biological Process
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: InterPro
Regulation of apoptotic process Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of neuron apoptotic process Source: UniProtKB
Cellular Location
Cytosol. Found in the cytosolic fraction of the brain.
Involvement in disease
Phosphorylated by AKT1 (PubMed:12524439). Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
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For research use only. Not intended for any clinical use.

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