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Mouse Anti-AKT1S1 Recombinant Antibody (V2-356450) (CBMAB-P0035-YC)

Provided herein is a Mouse monoclonal antibody against Human AKT1 Substrate 1. The antibody can be used for immunoassay techniques, such as WB, IP.
See all AKT1S1 antibodies
Published Data
Fig.1 Phosphorylation and protein expression of PRAS40 in A14 fibroblasts, L6 myotubes, and 3T3L1 adipocytes.
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Fig.2 Effect of RNA interference-induced PRAS40 depletion on the phosphorylation of endogenous S6K1 and 4E-BP1.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-356450
Antibody Isotype
IgG1
Application
WB, IP

Basic Information

Immunogen
Recombinant PRAS40 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT1 Substrate 1
Introduction
AKT1S1 is a proline-rich substrate of AKT that binds 14-3-3 protein when phosphorylated.
Entrez Gene ID
84335
UniProt ID
Q96B36
Alternative Names
1110012J22Rik; AI227026; Lobe; Lobel; PRAS40
Function
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Biological Process
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: InterPro
Regulation of apoptotic process Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of neuron apoptotic process Source: UniProtKB
Cellular Location
Cytosol. Found in the cytosolic fraction of the brain.
Involvement in disease
Phosphorylated by AKT1 (PubMed:12524439). Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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