Rabbit Anti-AKT1S1 Recombinant Antibody (V2-356451) (CBMAB-P0036-YC)

Name: Rabbit Anti-AKT1S1 Recombinant Antibody (V2-356451)
SKU: CBMAB-P0036-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-AKT1S1 Recombinant Antibody (D23C7) in IHC

mTORC1 and its downstream mediator are strongly activated in psoriatic lesions.
Punch biopsies from lesional psoriatic skin (a,c,e,g) of 20 patients and five healthy donors (b,d,f,h) were stained with antibodies for specific for Rheb, Raptor, P-PRAS40 and P-4E-BP. Nuclei were stained with DAPI. Representative overlay images from one patient and one healthy donor are shown. Bars represent 100 μm. ...View More

Buerger, C., Shirsath, N., Lang, V., Berard, A., Diehl, S., Kaufmann, R., ... & Wolf, P. (2017). Inflammation dependent mTORC1 signaling interferes with the switch from keratinocyte proliferation to differentiation. PLoS One, 12(7), e0180853.

Rabbit Anti-AKT1S1 Recombinant Antibody (D23C7) in IHC

Immunohistochemical evaluation of HPAC tumor xenografts treated with vehicle, GSK1120212 at 0.3 mg/kg QDx3 (MEK inh), GSK2141795 at 30 mg/kg QDx3 (AKT inh) or the combination of GSK1120212 plus GSK2141795 at 0.3 and 30 mg/kg, respectively (combo). Tumor tissues were stained with markers of proliferation (Ki67), apoptosis (Cleaved Caspase 3; CC3), MAPK pathway activation (phospho-ERK, dually phospho-ERK and total ERK) and AKT pathway activation (phospho-PRAS40, total PRAS40, phospho-S6 kinase, total S6K, phospho-AKT and total AKT). Quantitative image analysis of various immune-staining is represented as a percentage of the vehicle control (100%). Data represents mean ± s.d. ...View More

Dumble, M., Crouthamel, M. C., Zhang, S. Y., Schaber, M., Levy, D., Robell, K., ... & Kumar, R. (2014). Discovery of novel AKT inhibitors with enhanced anti-tumor effects in combination with the MEK inhibitor. PloS one, 9(6), e100880.

Rabbit Anti-AKT1S1 Recombinant Antibody (D23C7) in WB

A) Experimental design of tamoxifen treatment, body composition measurement, ITT, GTT, PTT, and clamp studies in control and AdHk2KO mice. Both control and AdHk2KO mice were treated with tamoxifen. (B) Immunoblot analyses of HK2 expression and insulin signaling in sWAT of control and adipose-specific Hk2 knockout (AdHk2KO) mice. Mice were fasted overnight or fasted overnight and re-fed for 3 hours. n=6. Source data Figure S3C. Quantification data is normalized to a loading control. (C) Immunoblot analyses of HK2 expression and insulin signaling in BAT of control and adipose-specific AdHk2KO mice. Mice were fasted overnight or fasted overnight and re-fed for 3 hours. n=6. Quantification data is normalized to a loading control. (D) Immunoblot analyses of HK2 expression and insulin signaling in skeletal muscle. of control and AdHk2KO mice. Mice were fasted overnight or fasted overnight and re-fed for 3 hours. n=7 (fasted control), 8 (refed control), 7 (fasted AdHk2KO), 8 (refed AdHk2KO). Quantification data is normalized to a loading control. ...View More

Shimobayashi, M., Thomas, A., Shetty, S., Frei, I. C., Wölnerhanssen, B. K., Weissenberger, D., ... & Hall, M. N. (2023). Diet-induced loss of adipose hexokinase 2 correlates with hyperglycemia. Elife, 12, e85103.

Rabbit Anti-AKT1S1 Recombinant Antibody (D23C7) in WB

hsa-miR-663a targeting PRAS40 and hsa-miR-1469 decrease phosphorylated p70S6K levels in HepG2 cells. HepG2 cells were transfected with 80 nM hsa-miR-663a and hsa-miR-1469 mimics for 48 h and analyzed using western blotting. (A) Representative western blot bands for PRAS40. (B) Quantification of panel (A). (C) Representative western blot bands for p70S6K and phosphorylated p70S6k. (D) Quantification of panel (C). Data are mean ± SE. *p < 0.05 and **p < 0.01, vs. Control. ...View More

Yamamura, J., Ma, S., Jia, H., & Kato, H. (2022). Activating transcription factor 4-dependent hsa-miR-663a transcription mediates mTORC1/p70S6K1 signaling underleucine deprivation. Frontiers in Nutrition, 9, 965771.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-356451

Application

WB, IHC-P, IP

Immunogen

Synthetic peptide corresponding to the sequence of human PRAS40.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50
IHC-P	1:800

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

AKT1 Substrate 1

Introduction

AKT1S1 is a proline-rich substrate of AKT that binds 14-3-3 protein when phosphorylated.

Entrez Gene ID

Human	84335
Mouse	67605
Rat	292887
Monkey	719101

UniProt ID

Human	Q96B36
Mouse	Q9D1F4
Rat	D3ZH75
Monkey	K7AMC0

Alternative Names

Lobe; PRAS40

Function

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Biological Process

Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Neurotrophin TRK receptor signaling pathway Source: InterPro
Regulation of apoptotic process Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of neuron apoptotic process Source: UniProtKB

Cellular Location

Cytosol. Found in the cytosolic fraction of the brain.

Involvement in disease

Phosphorylated by AKT1 (PubMed:12524439). Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1 (PubMed:23415227).

Wang, C., Chen, Y., Chen, K., & Zhang, L. (2020). Long noncoding RNA LINC01134 promotes hepatocellular carcinoma metastasis via activating AKT1S1 and NF-κB signaling. Frontiers in cell and developmental biology, 8, 429.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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