Mouse Anti-ALDH4A1 Recombinant Antibody (1A12-A5) (CBMAB-A2296-YC)

Name: Mouse Anti-ALDH4A1 Recombinant Antibody (1A12-A5)
SKU: CBMAB-A2296-YC
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Published Data

Mouse Anti-ALDH4A1 Recombinant Antibody (1A12-A5) in IHC and IF

Localization of ALDH4A1 in the postmortem brain. The localization of ALDH4A1 in the gray matter of the prefrontal cortex of the postmortem brain from healthy control. (A) The image of gray matter tissues immunostained for anti-ALDH4A1 antibodies.Immunopositive neuron was indicated by the arrow. Immnopositive astrocyte was indicated by the arrowhead. Immunopositive dots were scattered in the area of neuropil (circle). Scale bar, 20 μm. (B) Immunofluorescence images showed the location of ALDH4A1 (green) and mitochondria labeled with anti-COX antibodies (red). The arrowheads indicated the co-localization of ALDH4A1 with mitochondria. Scale bar, 20 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.) ...View More

Nagaoka, A., Kunii, Y., Hino, M., Izumi, R., Nagashima, C., Takeshima, A., ... & Yabe, H. (2020). ALDH4A1 expression levels are elevated in postmortem brains of patients with schizophrenia and are associated with genetic variants in enzymes related to proline metabolism. Journal of Psychiatric Research, 123, 119-127.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A12-A5

Application

ELISA, IHC, WB

Immunogen

Full length recombinant corresponding to aa1-564 from ALDH4A1 (AAH07581) with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-P	5 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 4 Family Member A1

Introduction

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. ALDH4A1 is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency

Entrez Gene ID

8659

UniProt ID

P30038

Alternative Names

Aldehyde Dehydrogenase 4 Family Member A1; L-Glutamate Gamma-Semialdehyde Dehydrogenase; Aldehyde Dehydrogenase Family 4 Member A1; P5C Dehydrogenase; ALDH4; P5CDh; Delta-1-Pyrroline-5-Carboxylate Dehydrogenase, Mitochondrial;

Function

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Biological Process

4-hydroxyproline catabolic process Source: BHF-UCL
Glyoxylate metabolic process Source: Reactome
Proline catabolic process Source: Reactome
Proline catabolic process to glutamate Source: UniProtKB-UniPathway
Proline metabolic process Source: ProtInc

Cellular Location

Mitochondrion matrix

Involvement in disease

Hyperprolinemia 2 (HYRPRO2): An inborn error of proline metabolism resulting in elevated plasma levels of proline and delta-1-pyrroline-5-carboxylate (P5C). The condition is considered to be benign, but affected individuals can exhibit neurological manifestations that vary in severity. Clinical signs include seizures, intellectual deficit and mild developmental delay.

Lorenzo, C., Delgado, P., Busse, C. E., Sanz-Bravo, A., Martos-Folgado, I., Bonzon-Kulichenko, E., ... & Ramiro, A. R. (2021). ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. Nature, 589(7841), 287-292.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Related Products

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Mouse Anti-ALDH4A1 Recombinant Antibody (F-1) (CAT#: CBMAB-A2297-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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