Human Recombinant ALDH4A1 protein, GST Tag (V2LY-0526-LY2001)

Name: Human Recombinant ALDH4A1 protein, GST Tag
SKU: V2LY-0526-LY2001
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ALDH4A1 protein, GST Tag consist of Amino Acid: 25-563 and predicts a molecular mass of 86.8 kDa.

Molecule Mass

86.8 kDa

Sequence

Amino Acid: 25-563

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 4 Family Member A1

Function

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Biological Process

4-hydroxyproline catabolic process Source: BHF-UCL
Glyoxylate metabolic process Source: Reactome
Proline catabolic process Source: Reactome
Proline catabolic process to glutamate Source: UniProtKB-UniPathway
Proline metabolic process Source: ProtInc

Cellular Location

Mitochondrion matrix

Involvement in disease

Hyperprolinemia 2 (HYRPRO2): An inborn error of proline metabolism resulting in elevated plasma levels of proline and delta-1-pyrroline-5-carboxylate (P5C). The condition is considered to be benign, but affected individuals can exhibit neurological manifestations that vary in severity. Clinical signs include seizures, intellectual deficit and mild developmental delay.

Lorenzo, C., Delgado, P., Busse, C. E., Sanz-Bravo, A., Martos-Folgado, I., Bonzon-Kulichenko, E., ... & Ramiro, A. R. (2021). ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. Nature, 589(7841), 287-292.

Nagaoka, A., Kunii, Y., Hino, M., Izumi, R., Nagashima, C., Takeshima, A., ... & Yabe, H. (2020). ALDH4A1 expression levels are elevated in postmortem brains of patients with schizophrenia and are associated with genetic variants in enzymes related to proline metabolism. Journal of psychiatric research, 123, 119-127.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Aldh4a1 ELISA Kit (CAT#: V2LY-0626-LY1794)

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Human Recombinant ALDH4A1 protein (CAT#: V2LY-0526-LY2000)

Antibody Pairs

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Related Products

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

Mouse Aldh4a1 ELISA Kit (CAT#: V2LY-0626-LY1794)

Human Recombinant ALDH4A1 protein (CAT#: V2LY-0526-LY2000)

Mouse Anti-ALDH4A1 Recombinant Antibody (F-1) (CAT#: CBMAB-A2297-YC)

Mouse Anti-ALDH4A1 Recombinant Antibody (1A12-A5) (CAT#: CBMAB-A2296-YC)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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