Mouse Anti-ALDH4A1 Recombinant Antibody (F-1) (CBMAB-A2297-YC)

Name: Mouse Anti-ALDH4A1 Recombinant Antibody (F-1)
SKU: CBMAB-A2297-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALDH4A1 Recombinant Antibody (F-1) in IF

Intracellular localization of IL21R in ADSCs and induced to adipogenesis. Immunofluorescence labeling of undifferentiated ADSCs using α-IL21R, α-ATP5B, and α-ALDH4A1 in a confocal microscope (a, b). ADSCs were incubated with the primary antibody α-IL21R and the secondary antibody conjugated to Alexa488. ADSCs were incubated with the primary antibody α-ATP5B (Atp5b ATP synthase located in the mitochondria) or ALDH4A1 and the secondary antibody conjugated to Alexa 546. ADSCs were incubated with DAPI for nuclear staining. (a, b) Images were then merged and showed that α-IL21R is colocalized with α-ATP5B and ALDH4A1, indicating that the protein is located in the mitochondria and within cell nuclei. (c, d) Localization of IL21R in premature adipocyte (c) and in adipocyte containing more lipid vesicles (d). Scale bar: 50 μm. Representative images of the donor TAL36 (a-d). ...View More

Falavinha, B. C., Barisón, M. J., Rebelatto, C. L. K., Marcon, B. H., de Melo Aguiar, A., da Silva, E. B., ... & Shigunov, P. (2022). Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells. Stem Cells International, 2022.

Mouse Anti-ALDH4A1 Recombinant Antibody (F-1) in Immunoblot

Representative immunoblots of ALDH1A1, ALDH1B1, ALDH2, and ALDH4A1 are shown with ERK2 as the loading control. Relative protein levels of all samples were determined by densitometry analysis normalized to ERK2 expression. Data are shown in box-whiskers graph (min to max value) (Normal, n = 8; steatosis, n = 4; NASH fatty, n = 11; NASH not fatty, n = 7). ...View More

Li, H., Toth, E., & Cherrington, N. J. (2018). Alcohol metabolism in the progression of human nonalcoholic steatohepatitis. Toxicological Sciences, 164(2), 428-438.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

F-1

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 261-288 within an internal region of ALDH4A1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 4 Family Member A1

Introduction

ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. ALDH4A1 is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency

Entrez Gene ID

Human	8659
Mouse	212647
Rat	641316

UniProt ID

Human	P30038
Mouse	Q8CHT0
Rat	P0C2X9

Alternative Names

Aldehyde Dehydrogenase 4 Family Member A1; L-Glutamate Gamma-Semialdehyde Dehydrogenase; Aldehyde Dehydrogenase Family 4 Member A1; P5C Dehydrogenase; ALDH4; P5CDh; Delta-1-Pyrroline-5-Carboxylate Dehydrogenase, Mitochondrial;

Function

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.

Biological Process

4-hydroxyproline catabolic process Source: BHF-UCL
Glyoxylate metabolic process Source: Reactome
Proline catabolic process Source: Reactome
Proline catabolic process to glutamate Source: UniProtKB-UniPathway
Proline metabolic process Source: ProtInc

Cellular Location

Mitochondrion matrix

Involvement in disease

Hyperprolinemia 2 (HYRPRO2): An inborn error of proline metabolism resulting in elevated plasma levels of proline and delta-1-pyrroline-5-carboxylate (P5C). The condition is considered to be benign, but affected individuals can exhibit neurological manifestations that vary in severity. Clinical signs include seizures, intellectual deficit and mild developmental delay.

Lorenzo, C., Delgado, P., Busse, C. E., Sanz-Bravo, A., Martos-Folgado, I., Bonzon-Kulichenko, E., ... & Ramiro, A. R. (2021). ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. Nature, 589(7841), 287-292.

Nagaoka, A., Kunii, Y., Hino, M., Izumi, R., Nagashima, C., Takeshima, A., ... & Yabe, H. (2020). ALDH4A1 expression levels are elevated in postmortem brains of patients with schizophrenia and are associated with genetic variants in enzymes related to proline metabolism. Journal of psychiatric research, 123, 119-127.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Related Products

Mouse Anti-ALDH4A1 Recombinant Antibody (1A12-A5) (CAT#: CBMAB-A2296-YC)

ALDH4A1 Matched Antibody Pair (065) (CAT#: APMAB-065LY)

ALDH4A1 Matched Antibody Pair (066) (CAT#: APMAB-066LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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