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Mouse Anti-AMY1A Recombinant Antibody (C-12) (CBMAB-A2605-YC)

Provided herein is a Mouse monoclonal antibody against Human Amylase, Alpha 1A (Salivary). The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all AMY1A antibodies
Published Data
Mouse Anti-AMY1A Recombinant Antibody (C-12) in WB
Effects of siTET2 on salivary gland functional proteins. PSGC kl +/+ cells were transfected with siTET2 for 48 hrs. After transfection, the protein levels of α-amylase, aqua5, and ZO-1 were analyzed by Western blotting. Actin was used as an internal control. ...View More
Toan, N. K., Kim, S. A., & Ahn, S. G. (2022). Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice. Aging (Albany NY), 14(15), 6028.
Mouse Anti-AMY1A Recombinant Antibody (C-12) in IF
Acinar lineage marker amylase following in vitro differentiation (n = 3, magnification: 40× or 60×, scale bar: 200 or 50 μM). ...View More
Zhang, F., Ma, D., Liu, T., Liu, Y. H., Guo, J., Song, J., ... & Jin, L. (2019). Expansion and maintenance of CD133-expressing pancreatic ductal epithelial cells by inhibition of TGF-β signaling. Stem Cells and Development, 28(18), 1236-1252.
Mouse Anti-AMY1A Recombinant Antibody (C-12) in IHC
The pancreatic exocrine marker amylase were detected in the graft (n = 7). Scale bar: 200 or 20 μM. ...View More
Zhang, F., Ma, D., Liu, T., Liu, Y. H., Guo, J., Song, J., ... & Jin, L. (2019). Expansion and maintenance of CD133-expressing pancreatic ductal epithelial cells by inhibition of TGF-β signaling. Stem Cells and Development, 28(18), 1236-1252.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
C-12
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 361-384 near the C-terminus of Amylase of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
amylase, alpha 1A (salivary)
Introduction
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are
Entrez Gene ID
Human276
Mouse11722
Rat24203
UniProt ID
HumanP04745
MouseP00687
RatQ99N59
Alternative Names
Amylase, Alpha 1A (Salivary); 1,4-Alpha-D-Glucan Glucanohydrolase 1; Salivary Alpha-Amylase; EC 3.2.1.1; AMY1; Amylase, Alpha 1A; Salivary;
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Biological Process
Oligosaccharide metabolic process Source: UniProtKB
Cellular Location
Secreted
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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