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Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) (CBMAB-A2606-YC)

Provided herein is a Mouse monoclonal antibody against Mouse Amylase, Alpha 1A (Salivary). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all AMY1A antibodies
Published Data
Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) in Immunoblot
Alpha-Amylase in BM (basketball male group), CM (control male group), BF (basketball female group), and CF (control female group). Normalization of immunoblot was performed on Coomassie-stained PVDF membrane. The statistical analysis was carried out by two-tailed t-test, using Graphpad Prism 6 (* p < 0.05; ** p < 0.01). ...View More
Luti, S., Militello, R., Pinto, G., Illiano, A., Amoresano, A., Chiappetta, G., ... & Gamberi, T. (2023, January). Chronic Training Induces Metabolic and Proteomic Response in Male and Female Basketball Players: Salivary Modifications during In-Season Training Programs. In Healthcare (Vol. 11, No. 2, p. 241). MDPI.
Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) in IHC
From the left side, representative images and corresponding quantification of ARID1A, CD45, amylase, MIST1, and cytokeratin 19 (CK19) staining performed in 8-week-old p48-Cre and Arid1afl/fl;p48-Cre mice. n = 5 mice/genotype. Scale bars: 100 μm. The quantitative evaluation of immunohistochemical staining was conducted with 5 mice/genotype by ImageJ version 1.53. (B and F) Data are presented as means ± SD and statistical significance was determined by a 2-tailed Mann-Whitney test. ∗P < .05, ∗∗P < .01. ...View More
Zhang, Z., Wang, X., Hamdan, F. H., Likhobabina, A., Patil, S., Aperdannier, L., ... & Hessmann, E. (2023). NFATc1 is a central mediator of EGFR-induced ARID1A chromatin dissociation during acinar cell reprogramming. Cellular and Molecular Gastroenterology and Hepatology, 15(5), 1219-1246.
Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) in IF
Immunofluorescence labeling of α-amylase and Ki-67 indicates less proliferating acinar cells in the Treg depleted group. Scale bars represent 20 µm. ...View More
Glaubitz, J., Wilden, A., Golchert, J., Homuth, G., Völker, U., Bröker, B. M., ... & Sendler, M. (2022). In mouse chronic pancreatitis CD25+ FOXP3+ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response. Nature Communications, 13(1), 4502.
Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) in IHC
H&E and IHC staining of paraffin sections of representative pancreata from KRASG12D/+ mice under ND, HFD, HCD, or HPD treatment for 10 weeks, using antibodies against amylase, CK19, and Muc5. Magnifications: 100× for H&E and 200× for IHC. ...View More
Zhu, L., Ji, J., Ma, J., Wang, D., Liu, M., Du, J. X., ... & Lu, W. (2022). Differential effects of dietary macronutrients on the development of oncogenic KRAS-mediated pancreatic ductal adenocarcinoma. Cancers, 14(11), 2723.
Mouse Anti-AMY1A Recombinant Antibody (CBYC-A571) in IF
hPG, hSMG, and hSLG tissues (left) and differentiated organoids (right) were subjected to IF staining for mucous (MUC7, green) and serous (AMY1, red) acinar cells. Scale bars indicate 50 μm. All data were representative of three independent experiments. Source Data are provided as a Source data file. ...View More
Yoon, Y. J., Kim, D., Tak, K. Y., Hwang, S., Kim, J., Sim, N. S., ... & Lim, J. Y. (2022). Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands. Nature communications, 13(1), 3291.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A571
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 212-492 of Amylase 2B of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 16-511

Target

Full Name
amylase, alpha 1A (salivary)
Introduction
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are
Entrez Gene ID
11722
UniProt ID
P00687
Alternative Names
Amylase, Alpha 1A (Salivary); 1,4-Alpha-D-Glucan Glucanohydrolase 1; Salivary Alpha-Amylase; EC 3.2.1.1; AMY1; Amylase, Alpha 1A; Salivary;
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Biological Process
Oligosaccharide metabolic process Source: UniProtKB
Cellular Location
Secreted
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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