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Mouse Anti-AMY1A Recombinant Antibody (CBXS-4491) (CBMAB-S1733-CQ)

This product is a mouse antibody that recognizes AMY1A. The antibody CBXS-4491 can be used for immunoassay techniques such as: WB, IHC-P.
See all AMY1A antibodies
Published Data
Mouse Anti-AMY1A Recombinant Antibody (CBXS-4491) in IF and IHC
H&E staining, TUNEL assay, and immunofluorescence of amylase, CK19, and SOX9. Black arrowheads, apoptotic cells; white arrowheads, CK19/amylase and SOX9/amylase double-positive cells; WT, wild-type; [Pft1a-CreERT-; LSL-TβRICA], CERR; [Pft1a-CreERT2; LSL-KrasG12D], CERK; [Pft1a-CreERT2; LSL-TβRICA; LSL-KrasG12D], CERKR. TAM, tamoxifen. Scale bars, 50 μm. ...View More
Chuvin, N., Vincent, D. F., Pommier, R. M., Alcaraz, L. B., Gout, J., Caligaris, C., ... & Bartholin, L. (2017). Acinar-to-ductal metaplasia induced by transforming growth factor beta facilitates KRASG12D-driven pancreatic tumorigenesis. Cellular and molecular gastroenterology and hepatology, 4(2), 263-282.
Mouse Anti-AMY1A Recombinant Antibody (CBXS-4491) in WB
Western blotting analysis of EV markers and nonEV proteins. ...View More
Li, M., Lou, D., Chen, J., Shi, K., Wang, Y., Zhu, Q., ... & Zhang, Y. (2021). Deep dive on the proteome of salivary extracellular vesicles: comparison between ultracentrifugation and polymer-based precipitation isolation. Analytical and bioanalytical chemistry, 413, 365-375.
Mouse Anti-AMY1A Recombinant Antibody (CBXS-4491) in IF
Paraffin section of a serous acinus. Triple channel for phase contrast (pc) and double immunofluorescence for amylase (red) and N-ANDV (green), Acinar cells contain amylase at the base and N-ANDV granules and masses in the supranuclear cytoplasm. n, cell nucleus; al, acinus lumen. ...View More
Pizarro, E., Navarrete, M., Mendez, C., Zaror, L., Mansilla, C., Tapia, M., ... & Rodríguez, E. M. (2020). Immunocytochemical and ultrastructural evidence supporting that Andes Hantavirus (ANDV) is transmitted person-to-person through the respiratory and/or salivary pathways. Frontiers in Microbiology, 10, 2992.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBXS-4491
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 385-417 near the C-terminus of Amylase of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
amylase, alpha 1A (salivary)
Introduction
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.
Entrez Gene ID
276
UniProt ID
P04745
Alternative Names
Amylase, Alpha 1A (Salivary); 1,4-Alpha-D-Glucan Glucanohydrolase 1; Salivary Alpha-Amylase; EC 3.2.1.1; AMY1; Amylase, Alpha 1A; Salivary;
Function
Calcium-binding enzyme that initiates starch digestion in the oral cavity (PubMed:12527308). Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds, yielding a mixture of maltose, isomaltose, small amounts of glucose as well as small linear and branched oligosaccharides called dextrins (PubMed:12527308).
Biological Process
Oligosaccharide metabolic process Source: UniProtKB
Cellular Location
Secreted
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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