Sign in or Register   Sign in or Register
  |  

Mouse Anti-API5 Recombinant Antibody (1C2) (CBMAB-A3069-YC)

Provided herein is a Mouse monoclonal antibody against Human Apoptosis Inhibitor 5. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all API5 antibodies
Published Data
Mouse Anti-API5 Recombinant Antibody (1C2) in IF
Endogenous Api5 (green) and E2F1 (red) immunostaining were performed on H1299 and HeLa cells; nuclei were stained with PI (blue). White arrows show cells undergoing mitosis. [Scale bar 20 µm]. ...View More
Garcia-Jove Navarro, M., Basset, C., Arcondéguy, T., Touriol, C., Perez, G., Prats, H., & Lacazette, E. (2013). Api5 contributes to E2F1 control of the G1/S cell cycle phase transition. PloS one, 8(8), e71443.

Summary

Host Animal
Mouse
Specificity
Human
Clone
1C2
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apoptosis Inhibitor 5
Introduction
API5 is an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor
Entrez Gene ID
8539
UniProt ID
Q9BZZ5
Alternative Names
Apoptosis Inhibitor 5; Fibroblast Growth Factor 2-Interacting Factor 2; Cell Migration-Inducing Gene 8 Protein; Migration-Inducing Protein MIG8; Antiapoptosis Clone 11 Protein; AAC-11; FIF;
Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Biological Process
Apoptotic process Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of fibroblast apoptotic process Source: Ensembl
Cellular Location
Cytoplasm; Nucleus. Mainly nuclear. Can also be cytoplasmic.
PTM
Acetylation at Lys-251 impairs antiapoptotic function.

Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., ... & Zhou, J. (2021). DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication. Mbio, 12(4), e01985-21.

Chen, M., Wu, W., Liu, D., Lv, Y., Deng, H., Gao, S., ... & Pang, Q. (2021). Evolution and Structure of API5 and Its Roles in Anti-Apoptosis. Protein and Peptide Letters, 28(6), 612-622.

Bong, S. M., Bae, S. H., Song, B., Gwak, H., Yang, S. W., Kim, S., ... & Lee, B. I. (2020). Regulation of mRNA export through API5 and nuclear FGF2 interaction. Nucleic acids research, 48(11), 6340-6352.

Imre, G., & Rajalingam, K. (2018). Apoptosis inhibitor 5: role in the inhibition of caspase-2. Cell Cycle, 17(4), 397-398.

Kim, Y. S., Park, H. J., Park, J. H., Hong, E. J., Jang, G. Y., Jung, I. D., ... & Park, Y. M. (2018). A novel function of API5 (apoptosis inhibitor 5), TLR4-dependent activation of antigen presenting cells. Oncoimmunology, 7(10), e1472187.

Imre, G., Berthelet, J., Heering, J., Kehrloesser, S., Melzer, I. M., Lee, B. I., ... & Rajalingam, K. (2017). Apoptosis inhibitor 5 is an endogenous inhibitor of caspase‐2. EMBO reports, 18(5), 733-744.

Zhang, S., Yao, F., Jing, T., Zhang, M., Zhao, W., Zou, X., ... & Hou, L. (2017). Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica. Gene, 628, 170-179.

Basset, C., Bonnet-Magnaval, F., Navarro, M. G. J., Touriol, C., Courtade, M., Prats, H., ... & Lacazette, E. (2017). Api5 a new cofactor of estrogen receptor alpha involved in breast cancer outcome. Oncotarget, 8(32), 52511.

Song, K. H., Cho, H., Kim, S., Lee, H. J., Oh, S. J., Woo, S. R., ... & Kim, T. W. (2017). API5 confers cancer stem cell-like properties through the FGF2-NANOG axis. Oncogenesis, 6(1), e285-e285.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-API5 Recombinant Antibody (1C2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare