Mouse Anti-API5 Recombinant Antibody (CBYC-A667) (CBMAB-A3070-YC)

Name: Mouse Anti-API5 Recombinant Antibody (CBYC-A667)
SKU: CBMAB-A3070-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A667

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Recombinant API5 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 400-504

More Infomation

Target

Full Name

Apoptosis Inhibitor 5

Introduction

API5 is an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor

Entrez Gene ID

8539

UniProt ID

Q9BZZ5

Alternative Names

Apoptosis Inhibitor 5; Fibroblast Growth Factor 2-Interacting Factor 2; Cell Migration-Inducing Gene 8 Protein; Migration-Inducing Protein MIG8; Antiapoptosis Clone 11 Protein; AAC-11; FIF;

Function

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Biological Process

Apoptotic process Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of fibroblast apoptotic process Source: Ensembl

Cellular Location

Cytoplasm; Nucleus. Mainly nuclear. Can also be cytoplasmic.

PTM

Acetylation at Lys-251 impairs antiapoptotic function.

Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., ... & Zhou, J. (2021). DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication. Mbio, 12(4), e01985-21.

Chen, M., Wu, W., Liu, D., Lv, Y., Deng, H., Gao, S., ... & Pang, Q. (2021). Evolution and Structure of API5 and Its Roles in Anti-Apoptosis. Protein and Peptide Letters, 28(6), 612-622.

Bong, S. M., Bae, S. H., Song, B., Gwak, H., Yang, S. W., Kim, S., ... & Lee, B. I. (2020). Regulation of mRNA export through API5 and nuclear FGF2 interaction. Nucleic acids research, 48(11), 6340-6352.

Imre, G., & Rajalingam, K. (2018). Apoptosis inhibitor 5: role in the inhibition of caspase-2. Cell Cycle, 17(4), 397-398.

Kim, Y. S., Park, H. J., Park, J. H., Hong, E. J., Jang, G. Y., Jung, I. D., ... & Park, Y. M. (2018). A novel function of API5 (apoptosis inhibitor 5), TLR4-dependent activation of antigen presenting cells. Oncoimmunology, 7(10), e1472187.

Imre, G., Berthelet, J., Heering, J., Kehrloesser, S., Melzer, I. M., Lee, B. I., ... & Rajalingam, K. (2017). Apoptosis inhibitor 5 is an endogenous inhibitor of caspase‐2. EMBO reports, 18(5), 733-744.

Zhang, S., Yao, F., Jing, T., Zhang, M., Zhao, W., Zou, X., ... & Hou, L. (2017). Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica. Gene, 628, 170-179.

Basset, C., Bonnet-Magnaval, F., Navarro, M. G. J., Touriol, C., Courtade, M., Prats, H., ... & Lacazette, E. (2017). Api5 a new cofactor of estrogen receptor alpha involved in breast cancer outcome. Oncotarget, 8(32), 52511.

Song, K. H., Cho, H., Kim, S., Lee, H. J., Oh, S. J., Woo, S. R., ... & Kim, T. W. (2017). API5 confers cancer stem cell-like properties through the FGF2-NANOG axis. Oncogenesis, 6(1), e285-e285.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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