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Mouse Anti-API5 Recombinant Antibody (EG198) (CBMAB-EN212-LY)

The product is antibody recognizes API5 . The antibody EG198 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000.
See all API5 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
EG198
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of API5 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Apoptosis Inhibitor 5
Introduction
This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Entrez Gene ID
Human8539
Mouse11800
UniProt ID
HumanQ9BZZ5
MouseO35841
Alternative Names
Apoptosis Inhibitor 5; Fibroblast Growth Factor 2-Interacting Factor 2; Cell Migration-Inducing Gene 8 Protein; Migration-Inducing Protein MIG8; Antiapoptosis Clone 11 Protein; AAC-11; FIF;
Function
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Biological Process
Apoptotic process Source: UniProtKB-KW
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of fibroblast apoptotic process Source: Ensembl
Cellular Location
Cytoplasm; Nucleus. Mainly nuclear. Can also be cytoplasmic.
PTM
Acetylation at Lys-251 impairs antiapoptotic function.

Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., ... & Zhou, J. (2021). DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication. Mbio, 12(4), e01985-21.

Chen, M., Wu, W., Liu, D., Lv, Y., Deng, H., Gao, S., ... & Pang, Q. (2021). Evolution and Structure of API5 and Its Roles in Anti-Apoptosis. Protein and Peptide Letters, 28(6), 612-622.

Bong, S. M., Bae, S. H., Song, B., Gwak, H., Yang, S. W., Kim, S., ... & Lee, B. I. (2020). Regulation of mRNA export through API5 and nuclear FGF2 interaction. Nucleic acids research, 48(11), 6340-6352.

Imre, G., & Rajalingam, K. (2018). Apoptosis inhibitor 5: role in the inhibition of caspase-2. Cell Cycle, 17(4), 397-398.

Kim, Y. S., Park, H. J., Park, J. H., Hong, E. J., Jang, G. Y., Jung, I. D., ... & Park, Y. M. (2018). A novel function of API5 (apoptosis inhibitor 5), TLR4-dependent activation of antigen presenting cells. Oncoimmunology, 7(10), e1472187.

Imre, G., Berthelet, J., Heering, J., Kehrloesser, S., Melzer, I. M., Lee, B. I., ... & Rajalingam, K. (2017). Apoptosis inhibitor 5 is an endogenous inhibitor of caspase‐2. EMBO reports, 18(5), 733-744.

Zhang, S., Yao, F., Jing, T., Zhang, M., Zhao, W., Zou, X., ... & Hou, L. (2017). Cloning, expression pattern, and potential role of apoptosis inhibitor 5 in the termination of embryonic diapause and early embryo development of Artemia sinica. Gene, 628, 170-179.

Basset, C., Bonnet-Magnaval, F., Navarro, M. G. J., Touriol, C., Courtade, M., Prats, H., ... & Lacazette, E. (2017). Api5 a new cofactor of estrogen receptor alpha involved in breast cancer outcome. Oncotarget, 8(32), 52511.

Song, K. H., Cho, H., Kim, S., Lee, H. J., Oh, S. J., Woo, S. R., ... & Kim, T. W. (2017). API5 confers cancer stem cell-like properties through the FGF2-NANOG axis. Oncogenesis, 6(1), e285-e285.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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