Mouse Anti-APLNR Recombinant Antibody (3C3) (CBMAB-A3080-YC)

Name: Mouse Anti-APLNR Recombinant Antibody (3C3)
SKU: CBMAB-A3080-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-APLNR Recombinant Antibody (3C3) in Immunoblot

The APLNR protein expression was evaluated with western blot in all of the samples. The immunoblot showed a strong band at approximately 35 Da. ...View More

Troisi, A., Dall'Aglio, C., Maranesi, M., Orlandi, R., Suvieri, C., Pastore, S., ... & Polisca, A. (2023). Presence and localization of apelin and its cognate receptor in canine testes using immunohistochemical and RT-PCR techniques. Veterinary Research Communications, 47(2), 929-935.

Mouse Anti-APLNR Recombinant Antibody (3C3) in IHC

Immunostaining for apelin (a) and apelin receptor (b) in the canine testes. Apelin (a) stained the residual bodies in the apical portion of the seminiferous portions (arrows). The apelin receptor (b) stained the basal line of spermatogenic epithelium (arrow) and Leydig cells in the connective tissue (asterisk). The image insets show the results of the negative controls performed to validate the IHC. ...View More

Mouse Anti-APLNR Recombinant Antibody (3C3) in IHC

APLN immunohistochemical aspects in the canine placenta and uterus: in a) immunopositivity is localized in the cytoplam of epithelial glandular cells (arrows); in b) immunopositivity is localized in the cytoplasm of epithelial lining cells (arrow); in c) immunopositivity is localized in some syncitiotrophoblasts of the placental labirintic portion (arrows). ...View More

Troisi, A., Dall'Aglio, C., Maranesi, M., Orlandi, R., Speranza, R., Zerani, M., & Polisca, A. (2020). Presence and expression of apelin and apelin receptor in bitch placenta. Theriogenology, 147, 192-196.

Mouse Anti-APLNR Recombinant Antibody (3C3) in IHC

Pig mandibular gland. APLNR binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups. In FP and CP samples, some cells (arrowhead) have a higher reactivity than others. ...View More

Dall'Aglio, C., Mercati, F., De Felice, E., Tardella, F. M., Kamphues, J., Cappai, M. G., & Scocco, P. (2020). Influence of different feed physical forms on mandibular gland in growing pigs. Animals, 10(5), 910.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3C3

Application

WB, IP, IF, IHC-P

Immunogen

Full-length recombinant APLNR of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Pig, Cattle

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apelin Receptor

Introduction

APLNR is a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressu

Entrez Gene ID

187

UniProt ID

P35414

Alternative Names

Apelin Receptor; G-Protein Coupled Receptor HG11; Angiotensin II Receptor-Like 1; Angiotensin Receptor-Like 1; APJ (Apelin) Receptor; AGTRL1; APJ;

Function

Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:11090199, PubMed:25639753, PubMed:28137936). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (PubMed:25639753, PubMed:28137936).
(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia (PubMed:11090199).

Biological Process

Adult heart development Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Blood vessel development Source: GO_Central
Coronary vasculature development Source: UniProtKB
Gastrulation Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: UniProtKB
Heart development Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: UniProtKB
Positive regulation of inhibitory G protein-coupled receptor phosphorylation Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Regulation of body fluid levels Source: Ensembl
Vasculogenesis Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Cell membrane; After exposure to apelin (APLN), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (By similarity). After exposure to apelin receptor early endogenous ligand (APELA), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (PubMed:25639753).

Topology

Extracellular: 1-26 aa
Helical: 27-51 aa
Cytoplasmic: 52-66 aa
Helical: 67-91 aa
Extracellular: 92-100 aa
Helical: 101-125 aa
Cytoplasmic: 126-144 aa
Helical: 145-166 aa
Extracellular: 167-200 aa
Helical: 201-221 aa
Cytoplasmic: 222-244 aa
Helical: 245-271 aa
Extracellular: 272-284 aa
Helical: 285-308 aa
Cytoplasmic: 309-380 aa

Saleme, B., Das, S. K., Zhang, Y., Boukouris, A. E., Lorenzana Carrillo, M. A., Jovel, J., ... & Sutendra, G. (2020). p53‐Mediated Repression of the PGC1A (PPARG Coactivator 1α) and APLNR (Apelin Receptor) Signaling Pathways Limits Fatty Acid Oxidation Energetics: Implications for Cardio‐oncology. Journal of the American Heart Association, 9(15), e017247.

Şişli, H. B., Hayal, T. B., Şenkal, S., Kıratlı, B., Sağraç, D., Seçkin, S., ... & Doğan, A. (2020). Apelin Receptor Signaling Protects GT1-7 GnRH Neurons Against Oxidative Stress In Vitro. Cellular and Molecular Neurobiology, 1-23.

Tolkach, Y., Ellinger, J., Kremer, A., Esser, L., Müller, S. C., Stephan, C., ... & Hauser, S. (2019). Apelin and apelin receptor expression in renal cell carcinoma. British journal of cancer, 120(6), 633-639.

Doğan, A. (2019). Apelin receptor (Aplnr) signaling promotes fibroblast migration. Tissue and Cell, 56, 98-106.

Zhang, J., Zhou, Y., Wu, C., Wan, Y., Fang, C., Li, J., ... & Wang, Y. (2018). Characterization of the apelin/elabela receptors (APLNR) in chickens, turtles, and zebrafish: identification of a novel apelin-specific receptor in teleosts. Frontiers in endocrinology, 9, 756.

Nowzari, Z., Masoumi, M., Nazari-Robati, M., Akbari, H., Shahrokhi, N., & Asadikaram, G. (2018). Association of polymorphisms of leptin, leptin receptor and apelin receptor genes with susceptibility to coronary artery disease and hypertension. Life sciences, 207, 166-171.

Hall, C., Ehrlich, L., Venter, J., O'Brien, A., White, T., Zhou, T., ... & Glaser, S. (2017). Inhibition of the apelin/apelin receptor axis decreases cholangiocarcinoma growth. Cancer letters, 386, 179-188.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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