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Rabbit Anti-APLNR Recombinant Antibody (5H5L9) (CBMAB-A3078-YC)

Provided herein is a Rabbit monoclonal antibody against Human Apelin Receptor. The antibody can be used for immunoassay techniques, such as ICC, IF, WB.
See all APLNR antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
5H5L9
Antibody Isotype
IgG
Application
ICC, IF, WB, IHC

Basic Information

Immunogen
Peptides corresponding to Human APLNR (aa 6-20, 52-66).
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-2 µg/ml
IF(ICC)2 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apelin Receptor
Introduction
APLNR is a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressu
Entrez Gene ID
Human187
Mouse23796
UniProt ID
HumanP35414
MouseQ9WV08
Alternative Names
Apelin Receptor; G-Protein Coupled Receptor HG11; Angiotensin II Receptor-Like 1; Angiotensin Receptor-Like 1; APJ (Apelin) Receptor; AGTRL1; APJ;
Function
Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:11090199, PubMed:25639753, PubMed:28137936). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone (By similarity). May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis (By similarity). Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development (By similarity). Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (PubMed:25639753, PubMed:28137936).
(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia (PubMed:11090199).
Biological Process
Adult heart development Source: Ensembl
Angiogenesis Source: UniProtKB-KW
Blood vessel development Source: GO_Central
Coronary vasculature development Source: UniProtKB
Gastrulation Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: UniProtKB
Heart development Source: UniProtKB
Negative regulation of cAMP-mediated signaling Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: UniProtKB
Positive regulation of inhibitory G protein-coupled receptor phosphorylation Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Regulation of body fluid levels Source: Ensembl
Vasculogenesis Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Cell membrane; After exposure to apelin (APLN), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (By similarity). After exposure to apelin receptor early endogenous ligand (APELA), internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane (PubMed:25639753).
Topology
Extracellular: 1-26 aa
Helical: 27-51 aa
Cytoplasmic: 52-66 aa
Helical: 67-91 aa
Extracellular: 92-100 aa
Helical: 101-125 aa
Cytoplasmic: 126-144 aa
Helical: 145-166 aa
Extracellular: 167-200 aa
Helical: 201-221 aa
Cytoplasmic: 222-244 aa
Helical: 245-271 aa
Extracellular: 272-284 aa
Helical: 285-308 aa
Cytoplasmic: 309-380 aa

Saleme, B., Das, S. K., Zhang, Y., Boukouris, A. E., Lorenzana Carrillo, M. A., Jovel, J., ... & Sutendra, G. (2020). p53‐Mediated Repression of the PGC1A (PPARG Coactivator 1α) and APLNR (Apelin Receptor) Signaling Pathways Limits Fatty Acid Oxidation Energetics: Implications for Cardio‐oncology. Journal of the American Heart Association, 9(15), e017247.

Şişli, H. B., Hayal, T. B., Şenkal, S., Kıratlı, B., Sağraç, D., Seçkin, S., ... & Doğan, A. (2020). Apelin Receptor Signaling Protects GT1-7 GnRH Neurons Against Oxidative Stress In Vitro. Cellular and Molecular Neurobiology, 1-23.

Tolkach, Y., Ellinger, J., Kremer, A., Esser, L., Müller, S. C., Stephan, C., ... & Hauser, S. (2019). Apelin and apelin receptor expression in renal cell carcinoma. British journal of cancer, 120(6), 633-639.

Doğan, A. (2019). Apelin receptor (Aplnr) signaling promotes fibroblast migration. Tissue and Cell, 56, 98-106.

Zhang, J., Zhou, Y., Wu, C., Wan, Y., Fang, C., Li, J., ... & Wang, Y. (2018). Characterization of the apelin/elabela receptors (APLNR) in chickens, turtles, and zebrafish: identification of a novel apelin-specific receptor in teleosts. Frontiers in endocrinology, 9, 756.

Nowzari, Z., Masoumi, M., Nazari-Robati, M., Akbari, H., Shahrokhi, N., & Asadikaram, G. (2018). Association of polymorphisms of leptin, leptin receptor and apelin receptor genes with susceptibility to coronary artery disease and hypertension. Life sciences, 207, 166-171.

Hall, C., Ehrlich, L., Venter, J., O'Brien, A., White, T., Zhou, T., ... & Glaser, S. (2017). Inhibition of the apelin/apelin receptor axis decreases cholangiocarcinoma growth. Cancer letters, 386, 179-188.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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