Mouse Anti-APOA4 Recombinant Antibody (1D6B6) (CBMAB-A3131-YC)

Name: Mouse Anti-APOA4 Recombinant Antibody (1D6B6)
SKU: CBMAB-A3131-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-APOA4 Recombinant Antibody (1D6B6) in WB

Representative WB images showed the expression of albumin, IgM, IgG, ApoA1, ApoA4, and ApoE. ...View More

Zhen, K., Wei, X., Zhi, Z., Zhang, S., Cui, L., Li, Y., ... & Zhang, H. (2022). Comparison of Different Isolation Methods for Plasma-Derived Extracellular Vesicles in Patients with Hyperlipidemia. Life, 12(11), 1942.

Mouse Anti-APOA4 Recombinant Antibody (1D6B6) in WB

Western blot analysis showing serum Apo-AIV and clusterin α-chain. Data obtained with three representative serum samples (each 10 μg protein) from PC patients (U-80, U-102, U-103) (P), BPD patients (U-57, U-72, U-74) (B), and control subjects (UC-1, UC-4, UC-7) (C) are shown. Data of quantitative densitometry are shown at the bottom (mean ± SD). ...View More

Kazuno, S., Fujimura, T., Fujime, M., Miura, Y., & Ueno, T. (2021). O-glycosylated clusterin as a sensitive marker for diagnosing early stages of prostate cancer. The Prostate, 81(3), 170-181.

Mouse Anti-APOA4 Recombinant Antibody (1D6B6) in WB

CHO cells that stably express either S1P1, S1P2, or S1P3 were stimulated with 100 nM of S1P preincubated with the same concentrations of recombinant ApoM, albumin, or ApoA4. After 5 min incubation, total cell lysates were prepared to examine the activation of ERK1/2 and Akt by Western blot analysis. ...View More

Obinata, H., Kuo, A., Wada, Y., Swendeman, S., Liu, C. H., Blaho, V. A., ... & Hla, T. (2019). Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM-and albumin-deficient mice. Journal of lipid research, 60(11), 1912-1921.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1D6B6

Application

WB, IP

Immunogen

Recombinant human ApoA4 protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Apolipoprotein A4

Introduction

Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted

Entrez Gene ID

Human	337
Mouse	11808
Rat	25080
Monkey	702424

UniProt ID

Human	P06727
Mouse	P06728
Rat	P02651
Monkey	F7FQN6

Alternative Names

Apolipoprotein A4; Apolipoprotein A-IV; Apo-AIV; ApoA-IV;

Function

May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.

Biological Process

Amyloid fibril formation Source: Reactome
Cholesterol biosynthetic process Source: GO_Central
Cholesterol efflux Source: BHF-UCL
Cholesterol homeostasis Source: BHF-UCL
Cholesterol metabolic process Source: BHF-UCL
Chylomicron assembly Source: BHF-UCL
Chylomicron remodeling Source: Reactome
High-density lipoprotein particle assembly Source: GO_Central
High-density lipoprotein particle remodeling Source: BHF-UCL
Hydrogen peroxide catabolic process Source: HGNC-UCL
Innate immune response in mucosa Source: BHF-UCL
Leukocyte cell-cell adhesion Source: BHF-UCL
Lipid catabolic process Source: BHF-UCL
Lipid homeostasis Source: BHF-UCL
Lipid transport Source: BHF-UCL
Lipoprotein metabolic process Source: GO_Central
Negative regulation of plasma lipoprotein oxidation Source: BHF-UCL
Phosphatidylcholine metabolic process Source: BHF-UCL
Phospholipid efflux Source: BHF-UCL
Positive regulation of cholesterol esterification Source: BHF-UCL
Positive regulation of fatty acid biosynthetic process Source: BHF-UCL
Positive regulation of lipid biosynthetic process Source: GO_Central
Positive regulation of lipoprotein lipase activity Source: BHF-UCL
Positive regulation of triglyceride catabolic process Source: BHF-UCL
Protein-lipid complex assembly Source: BHF-UCL
Regulation of cholesterol transport Source: BHF-UCL
Regulation of intestinal cholesterol absorption Source: GO_Central
Removal of superoxide radicals Source: HGNC-UCL
Response to lipid hydroperoxide Source: HGNC-UCL
Response to stilbenoid Source: Ensembl
Retinoid metabolic process Source: Reactome
Reverse cholesterol transport Source: BHF-UCL
Triglyceride homeostasis Source: GO_Central
Very-low-density lipoprotein particle remodeling Source: BHF-UCL

Cellular Location

Secreted

PTM

Phosphorylation sites are present in the extracellular medium.

Qu, J., Fourman, S., Fitzgerald, M., Liu, M., Nair, S., Oses-Prieto, J., ... & Bhargava, A. (2021). Low-density lipoprotein receptor-related protein 1 (LRP1) is a novel receptor for apolipoprotein A4 (APOA4) in adipose tissue. Scientific reports, 11(1), 1-14.

Li, X., Liu, X., Zhang, Y., Cheng, C., Fan, J., Zhou, J., ... & Li, Z. (2021). Hepatoprotective effect of apolipoprotein A4 against carbon tetrachloride induced acute liver injury through mediating hepatic antioxidant and inflammation response in mice. Biochemical and Biophysical Research Communications, 534, 659-665.

Li, M., Yang, X., Sun, L., Qing, Y., Hu, X., Jiang, J., ... & Wan, C. (2021). Decreased serum apolipoprotein A4 as a potential peripheral biomarker for patients with schizophrenia. Journal of Psychiatric Research, 137, 14-21.

Wang, Y., Yang, Z., Wei, Y., Li, X., & Li, S. (2021). Apolipoprotein A4 regulates the immune response in carbon tetrachloride-induced chronic liver injury in mice. International Immunopharmacology, 90, 107222.

Soukup, V., Capoun, O., Pesl, M., Vavrova, L., Sobotka, R., Levova, K., ... & Kalousova, M. (2019). The significance of calprotectin, CD147, APOA4 and DJ-1 in non-invasive detection of urinary bladder carcinoma. Neoplasma, 66(06), 1019-1023.

Kang, M., Kim, J., An, H. T., & Ko, J. (2017). Human leucine zipper protein promotes hepatic steatosis via induction of apolipoprotein A‐IV. The FASEB Journal, 31(6), 2548-2561.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

APOA4 Matched Antibody Pair (095) (CAT#: APMAB-095LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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