Mouse Apoa4 ELISA Kit (V2LY-0626-LY2045)

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Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
3.13 ng/mL
Detection Range
7-1500 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
45.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Apolipoprotein A4
Function
May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Biological Process
Amyloid fibril formation Source: Reactome
Cholesterol biosynthetic process Source: GO_Central
Cholesterol efflux Source: BHF-UCL
Cholesterol homeostasis Source: BHF-UCL
Cholesterol metabolic process Source: BHF-UCL
Chylomicron assembly Source: BHF-UCL
Chylomicron remodeling Source: Reactome
High-density lipoprotein particle assembly Source: GO_Central
High-density lipoprotein particle remodeling Source: BHF-UCL
Hydrogen peroxide catabolic process Source: HGNC-UCL
Innate immune response in mucosa Source: BHF-UCL
Leukocyte cell-cell adhesion Source: BHF-UCL
Lipid catabolic process Source: BHF-UCL
Lipid homeostasis Source: BHF-UCL
Lipid transport Source: BHF-UCL
Lipoprotein metabolic process Source: GO_Central
Negative regulation of plasma lipoprotein oxidation Source: BHF-UCL
Phosphatidylcholine metabolic process Source: BHF-UCL
Phospholipid efflux Source: BHF-UCL
Positive regulation of cholesterol esterification Source: BHF-UCL
Positive regulation of fatty acid biosynthetic process Source: BHF-UCL
Positive regulation of lipid biosynthetic process Source: GO_Central
Positive regulation of lipoprotein lipase activity Source: BHF-UCL
Positive regulation of triglyceride catabolic process Source: BHF-UCL
Protein-lipid complex assembly Source: BHF-UCL
Regulation of cholesterol transport Source: BHF-UCL
Regulation of intestinal cholesterol absorption Source: GO_Central
Removal of superoxide radicals Source: HGNC-UCL
Response to lipid hydroperoxide Source: HGNC-UCL
Response to stilbenoid Source: Ensembl
Retinoid metabolic process Source: Reactome
Reverse cholesterol transport Source: BHF-UCL
Triglyceride homeostasis Source: GO_Central
Very-low-density lipoprotein particle remodeling Source: BHF-UCL
Cellular Location
Secreted
PTM
Phosphorylation sites are present in the extracellular medium.

Qu, J., Fourman, S., Fitzgerald, M., Liu, M., Nair, S., Oses-Prieto, J., ... & Bhargava, A. (2021). Low-density lipoprotein receptor-related protein 1 (LRP1) is a novel receptor for apolipoprotein A4 (APOA4) in adipose tissue. Scientific reports, 11(1), 1-14.

Li, X., Liu, X., Zhang, Y., Cheng, C., Fan, J., Zhou, J., ... & Li, Z. (2021). Hepatoprotective effect of apolipoprotein A4 against carbon tetrachloride induced acute liver injury through mediating hepatic antioxidant and inflammation response in mice. Biochemical and Biophysical Research Communications, 534, 659-665.

Li, M., Yang, X., Sun, L., Qing, Y., Hu, X., Jiang, J., ... & Wan, C. (2021). Decreased serum apolipoprotein A4 as a potential peripheral biomarker for patients with schizophrenia. Journal of Psychiatric Research, 137, 14-21.

Wang, Y., Yang, Z., Wei, Y., Li, X., & Li, S. (2021). Apolipoprotein A4 regulates the immune response in carbon tetrachloride-induced chronic liver injury in mice. International Immunopharmacology, 90, 107222.

Obinata, H., Kuo, A., Wada, Y., Swendeman, S., Liu, C. H., Blaho, V. A., ... & Hla, T. (2019). Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM-and albumin-deficient mice. Journal of lipid research, 60(11), 1912-1921.

Soukup, V., Capoun, O., Pesl, M., Vavrova, L., Sobotka, R., Levova, K., ... & Kalousova, M. (2019). The significance of calprotectin, CD147, APOA4 and DJ-1 in non-invasive detection of urinary bladder carcinoma. Neoplasma, 66(06), 1019-1023.

Kang, M., Kim, J., An, H. T., & Ko, J. (2017). Human leucine zipper protein promotes hepatic steatosis via induction of apolipoprotein A‐IV. The FASEB Journal, 31(6), 2548-2561.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Apoa4 ELISA Kit (CAT#: V2LY-0626-LY2060)

Human Recombinant APOA4 protein, His Tag (CAT#: V2LY-0526-LY2140)

Human Recombinant APOA4 protein, His Tag-1 (CAT#: V2LY-0526-LY2141)

APOA4 Matched Antibody Pair (095) (CAT#: APMAB-095LY)

Antibody Pairs

APOA4 Matched Antibody Pair (095) (CAT#: APMAB-095LY)

Related Products

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Rat Apoa4 ELISA Kit (CAT#: V2LY-0626-LY2060)

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Mouse Anti-APOA4 Recombinant Antibody (1D4C11) (CAT#: CBMAB-A3130-YC)

Mouse Anti-APOA4 Recombinant Antibody (CBT166) (CAT#: V2LY-0625-LY3746)

Mouse Anti-APOA4 Recombinant Antibody (1D6B6) (CAT#: CBMAB-A3131-YC)

Mouse Anti-APOA4 Recombinant Antibody (CBT2645) (CAT#: V2LY-0625-LY2256)

Rabbit Anti-APOA4 Recombinant Antibody (13B34) (CAT#: CBMAB-A3128-YC)

Mouse Anti-APOA4 Recombinant Antibody (CL0465) (CAT#: CBMAB-A3133-YC)

APOA4 Matched Antibody Pair (095) (CAT#: APMAB-095LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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