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Mouse Anti-APOL1 Recombinant Antibody (1D4) (CBMAB-A3258-YC)

Provided herein is a Mouse monoclonal antibody against Human Apolipoprotein L1. The antibody can be used for immunoassay techniques, such as ELISA, WB, IHC-P, IF, ICC, FC.
See all APOL1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1D4
Antibody Isotype
IgG1
Application
WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of APOL1 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000
ELISA1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Ascites
Preservative
0.03% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Apolipoprotein L1
Introduction
APOL1 is a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also pr
Entrez Gene ID
8542
UniProt ID
O14791
Alternative Names
Apolipoprotein L1; Apolipoprotein L; APOL-I; APO-L; APOL; Apolipoprotein L1-B3; Apolipoprotein L 1; Apolipoprotein L-I; FSGS4;
Function
May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.
Biological Process
Cellular protein metabolic process Source: Reactome
Chloride transmembrane transport Source: BHF-UCL
Cholesterol metabolic process Source: UniProtKB-KW
Cytolysis by host of symbiont cells Source: BHF-UCL
Innate immune response Source: BHF-UCL
Lipid transport Source: UniProtKB-KW
Lipoprotein metabolic process Source: InterPro
Post-translational protein modification Source: Reactome
Receptor-mediated endocytosis Source: Reactome
Cellular Location
Secreted
Involvement in disease
Focal segmental glomerulosclerosis 4 (FSGS4): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
PTM
Phosphorylated by FAM20C in the extracellular medium.

Berrigan, M., Austrie, J., Fleishman, A., Tercyak, K. P., Pollak, M. R., Pavlakis, M., ... & Rodrigue, J. R. (2021). Opinions of African American adults about the use of apolipoprotein L1 (ApoL1) genetic testing in living kidney donation and transplantation. American Journal of Transplantation, 21(3), 1197-1205.

Scales, S. J., Gupta, N., De Mazière, A. M., Posthuma, G., Chiu, C. P., Pierce, A. A., ... & Peterson, A. S. (2020). Apolipoprotein L1-specific antibodies detect endogenous APOL1 inside the endoplasmic reticulum and on the plasma membrane of podocytes. Journal of the American Society of Nephrology, 31(9), 2044-2064.

Gordon, E. J., Wicklund, C., Lee, J., Sharp, R. R., & Friedewald, J. (2019). A national survey of transplant surgeons and nephrologists on implementing apolipoprotein L1 (APOL1) genetic testing into clinical practice. Progress in Transplantation, 29(1), 26-35.

Lannon, H., Shah, S. S., Dias, L., Blackler, D., Alper, S. L., Pollak, M. R., & Friedman, D. J. (2019). Apolipoprotein L1 (APOL1) risk variant toxicity depends on the haplotype background. Kidney international, 96(6), 1303-1307.

Young, B. A., Blacksher, E., Cavanaugh, K. L., Freedman, B. I., Fullerton, S. M., Kopp, J. B., ... & Burke, W. (2019). Apolipoprotein L1 testing in African Americans: involving the community in policy discussions. American journal of nephrology, 50(4), 303-311.

Kumar, V., Ayasolla, K., Jha, A., Mishra, A., Vashistha, H., Lan, X., ... & Singhal, P. C. (2019). Disrupted apolipoprotein L1-miR193a axis dedifferentiates podocytes through autophagy blockade in an APOL1 risk milieu. American Journal of Physiology-Cell Physiology, 317(2), C209-C225.

Freedman, B. I., Limou, S., Ma, L., & Kopp, J. B. (2018). APOL1-associated nephropathy: a key contributor to racial disparities in CKD. American Journal of Kidney Diseases, 72(5), S8-S16.

Kumar, V., Vashistha, H., Lan, X., Chandel, N., Ayasolla, K., Shoshtari, S. S. M., ... & Singhal, P. C. (2018). Role of apolipoprotein L1 in human parietal epithelial cell transition. The American journal of pathology, 188(11), 2508-2528.

Nadkarni, G. N., Galarneau, G., Ellis, S. B., Nadukuru, R., Zhang, J., Scott, S. A., ... & Bottinger, E. P. (2017). Apolipoprotein L1 variants and blood pressure traits in African Americans. Journal of the American College of Cardiology, 69(12), 1564-1574.

Bruno, J., Pozzi, N., Oliva, J., & Edwards, J. C. (2017). Apolipoprotein L1 confers pH-switchable ion permeability to phospholipid vesicles. Journal of Biological Chemistry, 292(44), 18344-18353.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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