Human Recombinant APOL1 protein, His Tag-1 (V2LY-0526-LY2143)

Name: Human Recombinant APOL1 protein, His Tag-1
SKU: V2LY-0526-LY2143
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant APOL1 protein, His Tag consist of Amino Acid: 1-398 and predicts a molecular mass of 42.53 kDa.

Molecule Mass

42.53 kDa

Sequence

Amino Acid: 1-398

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥80% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Apolipoprotein L1

Function

May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver.

Biological Process

Cellular protein metabolic process Source: Reactome
Chloride transmembrane transport Source: BHF-UCL
Cholesterol metabolic process Source: UniProtKB-KW
Cytolysis by host of symbiont cells Source: BHF-UCL
Innate immune response Source: BHF-UCL
Lipid transport Source: UniProtKB-KW
Lipoprotein metabolic process Source: InterPro
Post-translational protein modification Source: Reactome
Receptor-mediated endocytosis Source: Reactome

Cellular Location

Secreted

Involvement in disease

Focal segmental glomerulosclerosis 4 (FSGS4): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

PTM

Phosphorylated by FAM20C in the extracellular medium.

Berrigan, M., Austrie, J., Fleishman, A., Tercyak, K. P., Pollak, M. R., Pavlakis, M., ... & Rodrigue, J. R. (2021). Opinions of African American adults about the use of apolipoprotein L1 (ApoL1) genetic testing in living kidney donation and transplantation. American Journal of Transplantation, 21(3), 1197-1205.

Scales, S. J., Gupta, N., De Mazière, A. M., Posthuma, G., Chiu, C. P., Pierce, A. A., ... & Peterson, A. S. (2020). Apolipoprotein L1-specific antibodies detect endogenous APOL1 inside the endoplasmic reticulum and on the plasma membrane of podocytes. Journal of the American Society of Nephrology, 31(9), 2044-2064.

Gordon, E. J., Wicklund, C., Lee, J., Sharp, R. R., & Friedewald, J. (2019). A national survey of transplant surgeons and nephrologists on implementing apolipoprotein L1 (APOL1) genetic testing into clinical practice. Progress in Transplantation, 29(1), 26-35.

Lannon, H., Shah, S. S., Dias, L., Blackler, D., Alper, S. L., Pollak, M. R., & Friedman, D. J. (2019). Apolipoprotein L1 (APOL1) risk variant toxicity depends on the haplotype background. Kidney international, 96(6), 1303-1307.

Young, B. A., Blacksher, E., Cavanaugh, K. L., Freedman, B. I., Fullerton, S. M., Kopp, J. B., ... & Burke, W. (2019). Apolipoprotein L1 testing in African Americans: involving the community in policy discussions. American journal of nephrology, 50(4), 303-311.

Kumar, V., Ayasolla, K., Jha, A., Mishra, A., Vashistha, H., Lan, X., ... & Singhal, P. C. (2019). Disrupted apolipoprotein L1-miR193a axis dedifferentiates podocytes through autophagy blockade in an APOL1 risk milieu. American Journal of Physiology-Cell Physiology, 317(2), C209-C225.

Freedman, B. I., Limou, S., Ma, L., & Kopp, J. B. (2018). APOL1-associated nephropathy: a key contributor to racial disparities in CKD. American Journal of Kidney Diseases, 72(5), S8-S16.

Kumar, V., Vashistha, H., Lan, X., Chandel, N., Ayasolla, K., Shoshtari, S. S. M., ... & Singhal, P. C. (2018). Role of apolipoprotein L1 in human parietal epithelial cell transition. The American journal of pathology, 188(11), 2508-2528.

Nadkarni, G. N., Galarneau, G., Ellis, S. B., Nadukuru, R., Zhang, J., Scott, S. A., ... & Bottinger, E. P. (2017). Apolipoprotein L1 variants and blood pressure traits in African Americans. Journal of the American College of Cardiology, 69(12), 1564-1574.

Bruno, J., Pozzi, N., Oliva, J., & Edwards, J. C. (2017). Apolipoprotein L1 confers pH-switchable ion permeability to phospholipid vesicles. Journal of Biological Chemistry, 292(44), 18344-18353.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant APOL1 protein, His Tag (CAT#: V2LY-0526-LY2142)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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