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Mouse Anti-ARAF Recombinant Antibody (A5) (CBMAB-A3393-YC)


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Published Data
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
U2OS cells were transfected with a NC siRNA or an siRNA against HERC2. Levels of A-RAF was analysed by immunoblot, quantified, normalised based on CHC protein levels, and expressed relative to the control condition. ...View More
Sala-Gaston, J., Pedrazza, L., Ramirez, J., Martinez-Martinez, A., Rawlins, L. E., Baple, E. L., ... & Rosa, J. L. (2022). HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress. Cellular and Molecular Life Sciences, 79(11), 548.
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in IP
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in IP
In vitro kinase assays performed using inactive MEK1. Kinase activity (pMEK) was determined by western blot, and the pMEK/MEK ratio was quantified and normalized to immunoprecipitated RAF and parental DMSO-treated cells. ...View More
Yen, I., Shanahan, F., Lee, J., Hong, Y. S., Shin, S. J., Moore, A. R., ... & Malek, S. (2021). ARAF mutations confer resistance to the RAF inhibitor belvarafenib in melanoma. Nature, 594(7863), 418-423.
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
Lysates from U2OS cells transfected with the indicated siRNA (NT: non-targeting; Q1: HERC1; A: A-RAF; B: B-RAF; and C: C-RAF) were analyzed by immunoblotting with antibodies against the indicated proteins. MKK3 levels were quantified and normalized by CHC levels (n = 4). ...View More
Pedrazza, L., Schneider, T., Bartrons, R., Ventura, F., & Rosa, J. L. (2020). The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling. Scientific Reports, 10(1), 824.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A5
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IF, IP

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 145-365 of human ARAF (NP_001645) produced in E. coli
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
A-Raf Proto-Oncogene, Serine/Threonine Kinase
Introduction
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.
Entrez Gene ID
369
UniProt ID
P10398
Alternative Names
A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Thr
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Biological Process
Cellular protein modification process Source: ProtInc
MAPK cascade Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: UniProtKB
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB
Cellular Location
Cytosol; Mitochondrion
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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