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Mouse Anti-ARAF Recombinant Antibody (A5) (CBMAB-A3393-YC)

Provided herein is a Mouse monoclonal antibody against Human A-Raf Proto-Oncogene, Serine/Threonine Kinase. The antibody can be used for immunoassay techniques, such as WB.
See all ARAF antibodies
Published Data
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
U2OS cells were transfected with a NC siRNA or an siRNA against HERC2. Levels of A-RAF was analysed by immunoblot, quantified, normalised based on CHC protein levels, and expressed relative to the control condition. ...View More
Sala-Gaston, J., Pedrazza, L., Ramirez, J., Martinez-Martinez, A., Rawlins, L. E., Baple, E. L., ... & Rosa, J. L. (2022). HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress. Cellular and Molecular Life Sciences, 79(11), 548.
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in IP
In vitro kinase assays performed using inactive MEK1. Kinase activity (pMEK) was determined by western blot, and the pMEK/MEK ratio was quantified and normalized to immunoprecipitated RAF and parental DMSO-treated cells. ...View More
Yen, I., Shanahan, F., Lee, J., Hong, Y. S., Shin, S. J., Moore, A. R., ... & Malek, S. (2021). ARAF mutations confer resistance to the RAF inhibitor belvarafenib in melanoma. Nature, 594(7863), 418-423.
Mouse Anti-ARAF Recombinant Antibody (A5) CBMAB-A3393-YC in Immunoblot
Lysates from U2OS cells transfected with the indicated siRNA (NT: non-targeting; Q1: HERC1; A: A-RAF; B: B-RAF; and C: C-RAF) were analyzed by immunoblotting with antibodies against the indicated proteins. MKK3 levels were quantified and normalized by CHC levels (n = 4). ...View More
Pedrazza, L., Schneider, T., Bartrons, R., Ventura, F., & Rosa, J. L. (2020). The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling. Scientific Reports, 10(1), 824.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A5
Antibody Isotype
IgG1, κ
Application
ELISA, WB, IF, IP

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 145-365 of human ARAF (NP_001645) produced in E. coli
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
A-Raf Proto-Oncogene, Serine/Threonine Kinase
Introduction
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development.
Entrez Gene ID
369
UniProt ID
P10398
Alternative Names
A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Thr
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Biological Process
Cellular protein modification process Source: ProtInc
MAPK cascade Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: UniProtKB
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB
Cellular Location
Cytosol; Mitochondrion
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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