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Rabbit Anti-ARAF Recombinant Antibody (D2P9P) (CBMAB-CP2190-LY)

The product is antibody recognizes A-Raf. The antibody D2P9P immunoassay techniques such as: WB,IP.
See all ARAF antibodies
Published Data
Rabbit Anti-ARAF Recombinant Antibody (D2P9P) CBMAB-CP2190-LY in Immunoblot
Validation of ARAF, BRAF, and CRAF (also called RAF1) knockdown by immunoblotting in KRAS-mutant NSCLC H2009 cells expressing shRNAs targeting all three RAFs (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387) or a negative control (shRen.713). Indicated shRNAs were expressed from either LT3GEPIR or UT4GEPIR (pCF806) vectors at single-copy genomic integration. Cells were treated with dox (1,000 ng/mL) for 72 h to induce shRNA expression prior to harvest. ...View More
Amen, A. M., Loughran, R. M., Huang, C. H., Lew, R. J., Ravi, A., Guan, Y., ... & Fellmann, C. (2022). Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi. Cell Reports Methods, 2(7).

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
D2P9P
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Recombinant protein specific to a central region within human A-Raf protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
A-Raf Proto-Oncogene, Serine/Threonine Kinase
Entrez Gene ID
Human369
Mouse11836
Rat64363
UniProt ID
HumanP10398
MouseP04627
RatP14056
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Biological Process
Cellular protein modification process Source: ProtInc
MAPK cascade Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: UniProtKB
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB
Cellular Location
Cytosol; Mitochondrion
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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