Rabbit Anti-ARAF Recombinant Antibody (D2P9P) (CBMAB-CP2190-LY)

Name: Rabbit Anti-ARAF Recombinant Antibody (D2P9P)
SKU: CBMAB-CP2190-LY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-ARAF Recombinant Antibody (D2P9P) CBMAB-CP2190-LY in Immunoblot

Validation of ARAF, BRAF, and CRAF (also called RAF1) knockdown by immunoblotting in KRAS-mutant NSCLC H2009 cells expressing shRNAs targeting all three RAFs (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387) or a negative control (shRen.713). Indicated shRNAs were expressed from either LT3GEPIR or UT4GEPIR (pCF806) vectors at single-copy genomic integration. Cells were treated with dox (1,000 ng/mL) for 72 h to induce shRNA expression prior to harvest. ...View More

Amen, A. M., Loughran, R. M., Huang, C. H., Lew, R. J., Ravi, A., Guan, Y., ... & Fellmann, C. (2022). Endogenous spacing enables co-processing of microRNAs and efficient combinatorial RNAi. Cell Reports Methods, 2(7).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

D2P9P

Application

WB, IP

Immunogen

Recombinant protein specific to a central region within human A-Raf protein.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

A-Raf Proto-Oncogene, Serine/Threonine Kinase

Entrez Gene ID

Human	369
Mouse	11836
Rat	64363

UniProt ID

Human	P10398
Mouse	P04627
Rat	P14056

Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

Biological Process

Cellular protein modification process Source: ProtInc
MAPK cascade Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: UniProtKB
Regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB

Cellular Location

Cytosol; Mitochondrion

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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