Mouse Anti-AREG Recombinant Antibody (CAP174) (CBMAB-AP421LY)

Name: Mouse Anti-AREG Recombinant Antibody (CAP174)
SKU: CBMAB-AP421LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AREG Recombinant Antibody (CAP174) CBMAB-AP421LY in WB and ELISA

qPCR, Western blotting, and ELISA analysis revealed increased AREG mRNA and protein levels in keratin-deficient keratinocytes and increased Areg mRNA levels in KtyII2/2 mice. ...View More

Scheffschick, A., Kiritsi, D., & Magin, T. M. (2019). Keratin defects trigger the itch-inducing cytokine thymic stromal lymphopoietin through amphiregulin-epidermal growth factor receptor signaling. Journal of Allergy and Clinical Immunology, 144(6), 1719-1722.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CAP174

Application

ELISA, IF, IHC-P, WB

Immunogen

Amino acids 161-185 near the C-terminus of Amphiregulin of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Amphiregulin

Introduction

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Entrez Gene ID

374

UniProt ID

P15514

Alternative Names

Amphiregulin; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor; Amphiregulin B; AREGB; CRDGF; SDGF; AR;

Function

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

Biological Process

Cell-cell signaling Source: ProtInc
COPII vesicle coating Source: Reactome
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Epidermal growth factor receptor signaling pathway Source: BHF-UCL
G protein-coupled receptor signaling pathway Source: BHF-UCL
MAPK cascade Source: Reactome
Membrane organization Source: Reactome
Negative regulation of epidermal growth factor receptor signaling pathway Source: Reactome
Positive regulation of cell population proliferation Source: BHF-UCL
Positive regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Positive regulation of keratinocyte proliferation Source: GO_Central
Positive regulation of protein kinase B signaling Source: Reactome
Signal transduction Source: Reactome

Cellular Location

Membrane

Topology

Helical: 199-221 aa

Hachim, M. Y., Elemam, N. M., Ramakrishnan, R. K., Salameh, L., Olivenstein, R., Hachim, I. Y., ... & Hamoudi, R. (2020). Blood and salivary amphiregulin levels as biomarkers for asthma. Frontiers in Medicine, 7.

Todd, J. L., Kelly, F. L., Nagler, A., Banner, K., Pavlisko, E. N., Belperio, J. A., ... & Palmer, S. M. (2020). Amphiregulin contributes to airway remodeling in chronic allograft dysfunction after lung transplantation. American Journal of Transplantation, 20(3), 825-833.

Santamaría, E., Rodríguez‐Ortigosa, C. M., Uriarte, I., Latasa, M. U., Urtasun, R., Alvarez‐Sola, G., ... & Berasain, C. (2019). The epidermal growth factor receptor ligand amphiregulin protects from cholestatic liver injury and regulates bile acids synthesis. Hepatology, 69(4), 1632-1647.

Steponaitis, G., Kazlauskas, A., Skiriute, D., Vaitkiene, P., Skauminas, K., & Tamasauskas, A. (2019). Significance of amphiregulin (AREG) for the outcome of low and high grade astrocytoma patients. Journal of Cancer, 10(6), 1479.

Raimondo, S., Saieva, L., Vicario, E., Pucci, M., Toscani, D., Manno, M., ... & Alessandro, R. (2019). Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis. Journal of hematology & oncology, 12(1), 1-15.

Xu, Q., Long, Q., Zhu, D., Fu, D., Zhang, B., Han, L., ... & Sun, Y. (2019). Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD‐L1)‐mediated immunosuppression. Aging cell, 18(6), e13027.

Dogan, M., Sahin, M., & Yenisey, C. (2019). Increased TSLP, IL-33, IL-25, IL-19, IL 21 and amphiregulin (AREG) levels in chronic rhinosinusitis with nasal polyp. European Archives of Oto-Rhino-Laryngology, 276(6), 1685-1691.

Wang, J., Zhu, M., Wang, L., Chen, C., & Song, Y. (2019). Amphiregulin potentiates airway inflammation and mucus hypersecretion induced by urban particulate matter via the EGFR-PI3Kα-AKT/ERK pathway. Cellular signalling, 53, 122-131.

Vermillion, M. S., Ursin, R. L., Kuok, D. I., Vom Steeg, L. G., Wohlgemuth, N., Hall, O. J., ... & Klein, S. L. (2018). Production of amphiregulin and recovery from influenza is greater in males than females. Biology of sex differences, 9(1), 1-12.

Tung, S. L., Huang, W. C., Hsu, F. C., Yang, Z. P., Jang, T. H., Chang, J. W., ... & Wang, L. H. (2017). miRNA-34c-5p inhibits amphiregulin-induced ovarian cancer stemness and drug resistance via downregulation of the AREG-EGFR-ERK pathway. Oncogenesis, 6(5), e326-e326.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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