Mouse Anti-ARFGAP1 Recombinant Antibody (C4) (CBMAB-A3444-YC)

Provided herein is a Mouse monoclonal antibody against Human ADP Ribosylation Factor GTPase Activating Protein 1. The antibody can be used for immunoassay techniques, such as FC, IF, IHC-P, WB.
See all ARFGAP1 antibodies

Published Data

Mouse Anti-ARFGAP1 Recombinant Antibody (C4) CBMAB-A3444-YC in IF

Phagosomal acquisition of ArfGAP1 to phagosomes. Shown are images acquired by confocal microscopy using specific antibodies (in green) against the indicated proteins during phagosome maturation comparing resting and LPS-treated DCs. OVAcoupled beads are shown in red and F-actin in gray. ...View More

Pauwels, A. M., Härtlova, A., Peltier, J., Driege, Y., Baudelet, G., Brodin, P., ... & Hoffmann, E. (2019). Spatiotemporal changes of the phagosomal proteome in dendritic cells in response to LPS stimulation. Molecular & Cellular Proteomics, 18(5), 909-922.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

Antibody Isotype

IgG1, κ

Application

ELISA, IF, IP, WB

Basic Information

Immunogen

Amino acids 1-286 mapping at the N-terminus of ARFGAP1 of human origin.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ADP Ribosylation Factor GTPase Activating Protein 1

Introduction

ARFGAP1 is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of co

Entrez Gene ID

55738

UniProt ID

Q8N6T3

Alternative Names

ADP Ribosylation Factor GTPase Activating Protein 1; ARF1-Directed GTPase-Activating Protein; ARF1GAP; ADP-Ribosylation Factor 1 GTPase Activating Protein; ADP-Ribosylation Factor GTPase-Activating Protein 1; ADP-Ribosylation Factor 1 GTPase-Activating Pr

Function

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

Biological Process

Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
IRE1-mediated unfolded protein response Source: Reactome
Protein transport Source: UniProtKB-KW
Regulation of ARF protein signal transduction Source: GO_Central
Regulation of endocytosis Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome

Cellular Location

Cytoplasm; Golgi apparatus. Associates with the Golgi complex.

References

Feng, H. P., Cheng, H. Y., Hsiao, T. F., Lin, T. W., Hsu, J. W., Huang, L. H., & Yu, C. J. (2021). ArfGAP1 acts as a GTPase‐activating protein for human ADP‐ribosylation factor‐like 1 protein. The FASEB Journal, 35(4), e21337.

Luo, C., Peng, W., Kang, J., Chen, C., Peng, J., Wang, Y., ... & Pan, X. (2021). Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells. Journal of Agricultural and Food Chemistry.

Meng, D., Yang, Q., Melick, C. H., Park, B. C., Hsieh, T. S., Curukovic, A., ... & Jewell, J. L. (2021). ArfGAP1 inhibits mTORC1 lysosomal localization and activation. The EMBO Journal, 40(12), e106412.

Zhang, L., Jin, M., Song, M., Liu, S., Wang, T., Guo, K., & Zhang, Y. (2021). ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. Veterinary Microbiology, 255, 109034.

Song, O. R., Queval, C. J., Iantomasi, R., Delorme, V., Marion, S., Veyron‐Churlet, R., ... & Brodin, P. (2018). Arf GAP 1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton. EMBO reports, 19(1), 29-42.

Quartino, P. Y., Fidelio, G. D., Manneville, J. B., Goud, B., & Ambroggio, E. E. (2018). Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1. Biochemical and biophysical research communications, 505(1), 290-294.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-ARFGAP1 Recombinant Antibody (D4C2M)

Mouse Anti-ARFGAP1 Recombinant Antibody (1C4E2)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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