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Rabbit Anti-ARFGAP1 Recombinant Antibody (D4C2M) (CBMAB-A3449-YC)

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Basic Information

Host Animal
Rabbit
Clone
D4C2M
Application
WB, IP
Immunogen
Synthetic peptide corresponding to residues surrounding Gly177 of human ARFGAP1 protein.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
ADP Ribosylation Factor GTPase Activating Protein 1
Introduction
ARFGAP1 is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of co
Entrez Gene ID
Human55738
Mouse228998
Rat246310
UniProt ID
HumanQ8N6T3
MouseQ9EPJ9
RatQ62848
Alternative Names
ADP Ribosylation Factor GTPase Activating Protein 1; ARF1-Directed GTPase-Activating Protein; ARF1GAP; ADP-Ribosylation Factor 1 GTPase Activating Protein; ADP-Ribosylation Factor GTPase-Activating Protein 1; ADP-Ribosylation Factor 1 GTPase-Activating Pr
Function
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
Biological Process
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
IRE1-mediated unfolded protein response Source: Reactome
Protein transport Source: UniProtKB-KW
Regulation of ARF protein signal transduction Source: GO_Central
Regulation of endocytosis Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Cellular Location
Cytoplasm; Golgi apparatus. Associates with the Golgi complex.

Feng, H. P., Cheng, H. Y., Hsiao, T. F., Lin, T. W., Hsu, J. W., Huang, L. H., & Yu, C. J. (2021). ArfGAP1 acts as a GTPase‐activating protein for human ADP‐ribosylation factor‐like 1 protein. The FASEB Journal, 35(4), e21337.

Luo, C., Peng, W., Kang, J., Chen, C., Peng, J., Wang, Y., ... & Pan, X. (2021). Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells. Journal of Agricultural and Food Chemistry.

Meng, D., Yang, Q., Melick, C. H., Park, B. C., Hsieh, T. S., Curukovic, A., ... & Jewell, J. L. (2021). ArfGAP1 inhibits mTORC1 lysosomal localization and activation. The EMBO Journal, 40(12), e106412.

Zhang, L., Jin, M., Song, M., Liu, S., Wang, T., Guo, K., & Zhang, Y. (2021). ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. Veterinary Microbiology, 255, 109034.

Song, O. R., Queval, C. J., Iantomasi, R., Delorme, V., Marion, S., Veyron‐Churlet, R., ... & Brodin, P. (2018). Arf GAP 1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton. EMBO reports, 19(1), 29-42.

Quartino, P. Y., Fidelio, G. D., Manneville, J. B., Goud, B., & Ambroggio, E. E. (2018). Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1. Biochemical and biophysical research communications, 505(1), 290-294.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-ARFGAP1 Recombinant Antibody (CBT3044) (CAT#: V2LY-0625-LY620)

Mouse Anti-ARFGAP1 Recombinant Antibody (C4) (CAT#: CBMAB-A3444-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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