Mouse Anti-ARFGAP1 Recombinant Antibody (CBT3044) (V2LY-0625-LY620)

Name: Mouse Anti-ARFGAP1 Recombinant Antibody (CBT3044)
SKU: V2LY-0625-LY620
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

CBT3044

Antibody Isotype

IgG2b

Application

WB, FC

Basic Information

Immunogen

Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ADP Ribosylation Factor GTPase Activating Protein 1

Entrez Gene ID

55738

UniProt ID

Q8N6T3

Function

GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).

Biological Process

Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
IRE1-mediated unfolded protein response Source: Reactome
Protein transport Source: UniProtKB-KW
Regulation of ARF protein signal transduction Source: GO_Central
Regulation of endocytosis Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome

Cellular Location

Cytoplasm; Golgi apparatus. Associates with the Golgi complex.

More Infomation

References

Feng, H. P., Cheng, H. Y., Hsiao, T. F., Lin, T. W., Hsu, J. W., Huang, L. H., & Yu, C. J. (2021). ArfGAP1 acts as a GTPase‐activating protein for human ADP‐ribosylation factor‐like 1 protein. The FASEB Journal, 35(4), e21337.

Luo, C., Peng, W., Kang, J., Chen, C., Peng, J., Wang, Y., ... & Pan, X. (2021). Glutamine Regulates Cell Growth and Casein Synthesis through the CYTHs/ARFGAP1-Arf1-mTORC1 Pathway in Bovine Mammary Epithelial Cells. Journal of Agricultural and Food Chemistry.

Meng, D., Yang, Q., Melick, C. H., Park, B. C., Hsieh, T. S., Curukovic, A., ... & Jewell, J. L. (2021). ArfGAP1 inhibits mTORC1 lysosomal localization and activation. The EMBO Journal, 40(12), e106412.

Zhang, L., Jin, M., Song, M., Liu, S., Wang, T., Guo, K., & Zhang, Y. (2021). ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells. Veterinary Microbiology, 255, 109034.

Song, O. R., Queval, C. J., Iantomasi, R., Delorme, V., Marion, S., Veyron‐Churlet, R., ... & Brodin, P. (2018). Arf GAP 1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton. EMBO reports, 19(1), 29-42.

Quartino, P. Y., Fidelio, G. D., Manneville, J. B., Goud, B., & Ambroggio, E. E. (2018). Detecting phospholipase activity with the amphipathic lipid packing sensor motif of ArfGAP1. Biochemical and biophysical research communications, 505(1), 290-294.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Mouse Anti-ARFGAP1 Recombinant Antibody (C4)

Rabbit Anti-ARFGAP1 Recombinant Antibody (D4C2M)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

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