Mouse Anti-ARFGAP3 Recombinant Antibody (EG223) (CBMAB-EN250-LY)

The product is antibody recognizes ARFGAP3 . The antibody EG223 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all ARFGAP3 antibodies

Published Data

Mouse Anti-ARFGAP3 Recombinant Antibody (EG223) CBMAB-EN250-LY in IF

Accumulation of Ras family proteins in membrane protrusions. (A) Confocal images show the enrichment of different Ras proteins (green) (RasL12, Braf and Raf) in tumor cell blebs compared to the cytoplasmic region. Rasgrp3 was present in membrane blebs but was more abundant in the cytoplasm. (B) HAMLET (red) was also present in the membrane blebs. (C) Merged confocal images. Red lines represent intensity distribution region used for calculating Pearson coefficient. R = Pearson coefficient for co-localization. Scale bar, 5 m. ...View More

Nadeem, A., Sanborn, J., Gettel, D. L., James, H. C., Rydström, A., Ngassam, V. N., ... & Svanborg, C. (2015). Protein receptor-independent plasma membrane remodeling by HAMLET: a tumoricidal protein-lipid complex. Scientific reports, 5(1), 16432.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

EG223

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 139-177 near the N-terminus of ARFGAP1/3 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ADP Ribosylation Factor GTPase Activating Protein 3

Introduction

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]

Entrez Gene ID

26286

UniProt ID

Q9NP61

Alternative Names

ADP Ribosylation Factor GTPase Activating Protein 3; ADP-Ribosylation Factor GTPase Activating Protein 1; ARF GAP 3; ARFGAP1; ADP-Ribosylation Factor GTPase-Activating Protein 3;

Function

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Biological Process

COPI coating of Golgi vesicle Source: GO_Central
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Intracellular protein transport Source: UniProtKB
Protein secretion Source: UniProtKB
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Vesicle-mediated transport Source: UniProtKB

Cellular Location

Golgi apparatus membrane; Cytoplasm. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.

References

Sun, Y., Li, B., Lu, D., Liu, C., Hong, S., & Hong, L. (2021). Expression of ArfGAP3 in Vaginal Anterior Wall of Patients With Pelvic Floor Organ Prolapse in Pelvic Organ Prolapse and Non–Pelvic Organ Prolapse Patients. Female pelvic medicine & reconstructive surgery, 27(1), e64.

Muungo, L. (2020). ARFGAP3 an androgen target gene promotes prostate cancer cell proliferation and migration.

Yi, Y., Wang, L., Li, S., Li, B., Liu, C., & Hong, L. (2020). Effects of mechanical trauma on the differentiation and ArfGAP3 expression of C2C12 myoblast and mouse levator ani muscle. International urogynecology journal, 31(9), 1913-1924.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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