Human ARFGAP3 ELISA Kit (V2LY-0626-LY5753)

Name: Human ARFGAP3 ELISA Kit
SKU: V2LY-0626-LY5753
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0022 ng/mL

Detection Range

0.005-1.8 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

56.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

ADP Ribosylation Factor GTPase Activating Protein 3

Function

GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.

Biological Process

COPI coating of Golgi vesicle Source: GO_Central
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: Reactome
Intracellular protein transport Source: UniProtKB
Protein secretion Source: UniProtKB
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: Reactome
Vesicle-mediated transport Source: UniProtKB

Cellular Location

Golgi apparatus membrane; Cytoplasm. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.

Sun, Y., Li, B., Lu, D., Liu, C., Hong, S., & Hong, L. (2021). Expression of ArfGAP3 in Vaginal Anterior Wall of Patients With Pelvic Floor Organ Prolapse in Pelvic Organ Prolapse and Non–Pelvic Organ Prolapse Patients. Female pelvic medicine & reconstructive surgery, 27(1), e64.

Muungo, L. (2020). ARFGAP3 an androgen target gene promotes prostate cancer cell proliferation and migration.

Yi, Y., Wang, L., Li, S., Li, B., Liu, C., & Hong, L. (2020). Effects of mechanical trauma on the differentiation and ArfGAP3 expression of C2C12 myoblast and mouse levator ani muscle. International urogynecology journal, 31(9), 1913-1924.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ARFGAP3 Recombinant Antibody (EG223) (CAT#: CBMAB-EN250-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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