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Rabbit Anti-ARHGAP42 (Phosphorylated Y376) Recombinant Antibody (D45E9) (PTM-CBMAB-0044YC)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Monkey
Clone
D45E9
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein.
Specificity
Human, Mouse, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Rho GTPase Activating Protein 42
Introduction
ARHGAP42 is a Rho GTPase-activating protein (RhoGAP), and member of the GRAF or BAR-PH family of proteins. Expression of this gene is enriched in vascular smooth muscle cells and the encoded protein inhibits RhoA activity to regulate vascular tone and control blood pressure. A mutation in the first intron of this gene modulates its expression and is associated with reduced blood pressure in human patients with borderline hypertension.
Entrez Gene ID
Human143872
Mouse71544
Rat500943
Monkey703141
UniProt ID
HumanA6NI28
MouseB2RQE8
RatF1M2Q1
MonkeyH2Q4L7
Alternative Names
Rho GTPase Activating Protein 42; Rho-Type GTPase-Activating Protein 42; Transmembrane Protein 133; GRAF3; Rho GTPase-Activating Protein 10-Like; Rho GTPase-Activating Protein 42; TMEM133; AD031;
Function
May influence blood pressure by functioning as a GTPase-activating protein for RHOA in vascular smooth muscle.
Biological Process
Activation of GTPase activity Source: UniProtKB
Negative regulation of Rho protein signal transduction Source: Ensembl
Negative regulation of systemic arterial blood pressure Source: Ensembl
Negative regulation of vascular associated smooth muscle contraction Source: Ensembl
Signal transduction Source: InterPro
More Infomation

Li, Q., Dibus, M., Casey, A., Yee, C. S., Vargas, S. O., Luo, S., ... & Agrawal, P. B. (2021). A homozygous stop-gain variant in ARHGAP42 is associated with childhood interstitial lung disease, systemic hypertension, and immunological findings. PLoS genetics, 17(7), e1009639.

Mangum, K. D., Freeman, E. J., Magin, J. C., Taylor, J. M., & Mack, C. P. (2020). Transcriptional and posttranscriptional regulation of the SMC-selective blood pressure-associated gene, ARHGAP42. American Journal of Physiology-Heart and Circulatory Physiology, 318(2), H413-H424.

Fjorder, A. S., Rasmussen, M. B., Mehrjouy, M. M., Nazaryan-Petersen, L., Hansen, C., Bak, M., ... & Bache, I. (2019). Haploinsufficiency of ARHGAP42 is associated with hypertension. European Journal of Human Genetics, 27(8), 1296-1303.

Hu, Q., Lin, X., Ding, L., Zeng, Y., Pang, D., Ouyang, N., ... & Yao, H. (2018). ARHGAP 42 promotes cell migration and invasion involving PI 3K/Akt signaling pathway in nasopharyngeal carcinoma. Cancer medicine, 7(8), 3862-3874.

Luo, W., Janoštiak, R., Tolde, O., Ryzhova, L. M., Koudelková, L., Dibus, M., ... & Rosel, D. (2017). ARHGAP42 is activated by Src-mediated tyrosine phosphorylation to promote cell motility. Journal of cell science, 130(14), 2382-2393.

Bai, X., Mangum, K. D., Dee, R. A., Stouffer, G. A., Lee, C. R., Oni-Orisan, A., ... & Mack, C. P. (2017). Blood pressure–associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding. The Journal of clinical investigation, 127(2), 670-680.

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For research use only. Not intended for any clinical use.

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