Mouse Anti-ARHGEF5 Recombinant Antibody (3A12-B5) (CBMAB-A0470-LY)

Name: Mouse Anti-ARHGEF5 Recombinant Antibody (3A12-B5)
SKU: CBMAB-A0470-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3A12-B5

Application

WB, ELISA

Immunogen

ARHGEF5 (AAH14555, 1 a.a. ~ 519 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Rho Guanine Nucleotide Exchange Factor 5

Introduction

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq]

Entrez Gene ID

7984

UniProt ID

Q12774

Alternative Names

DKFZp686N1969; GEF5; P60; TIM; TIM1

Function

Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624).
Strongly activates RHOA (PubMed:15601624).
Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity).
Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624).
Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653).
Required for SRC-induced podosome formation (By similarity).
Involved in positive regulation of immature dendritic cell migration (By similarity).

Biological Process

Activation of GTPase activity Source: GO_Central
G protein-coupled receptor signaling pathway Source: Reactome
Intracellular signal transduction Source: InterPro
Positive regulation of apoptotic process Source: Reactome
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of JUN kinase activity Source: UniProtKB
Positive regulation of protein import Source: UniProtKB
Positive regulation of stress fiber assembly Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cytoskeleton organization Source: UniProtKB
Regulation of GTPase activity Source: MGI
Regulation of small GTPase mediated signal transduction Source: Reactome

Cellular Location

Cytoplasm; Nucleus; Podosome

PTM

Activation of SRC induces tyrosine phosphorylation of ARHGEF5.

Bernadzki, K. M., Daszczuk, P., Rojek, K. O., Pęziński, M., Gawor, M., Pradhan, B. S., ... & Niewiadomski, P. (2020). Arhgef5 binds α-dystrobrevin 1 and regulates neuromuscular junction integrity. Frontiers in Molecular Neuroscience, 13, 104.

Fu, Y., He, P., Zhou, Y., Huang, S., Liang, L., & Liu, S. (2019). Exploring the systematic effect of N‐substituted PxxP motifs on peptoid affinity to ARHGEF5/TIM SH3 domain and its relationship with ARHGEF5/TIM activation. Proteins: Structure, Function, and Bioinformatics, 87(11), 979-991.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ARHGEF5 Matched Antibody Pair (105) (CAT#: APMAB-105LY)

Related Products

ARHGEF5 Matched Antibody Pair (105) (CAT#: APMAB-105LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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