Human ARHGEF5 ELISA Kit (V2LY-0626-LY3708)

See all ARHGEF5 antibodies
Go to compare Compare Online Inquiry
Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.004 ng/mL
Detection Range
0.007-1.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
176.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Rho Guanine Nucleotide Exchange Factor 5
Function
Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624).
Strongly activates RHOA (PubMed:15601624).
Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity).
Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624).
Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653).
Required for SRC-induced podosome formation (By similarity).
Involved in positive regulation of immature dendritic cell migration (By similarity).
Biological Process
Activation of GTPase activity Source: GO_Central
G protein-coupled receptor signaling pathway Source: Reactome
Intracellular signal transduction Source: InterPro
Positive regulation of apoptotic process Source: Reactome
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of JUN kinase activity Source: UniProtKB
Positive regulation of protein import Source: UniProtKB
Positive regulation of stress fiber assembly Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cytoskeleton organization Source: UniProtKB
Regulation of GTPase activity Source: MGI
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Podosome
PTM
Activation of SRC induces tyrosine phosphorylation of ARHGEF5.

Bernadzki, K. M., Daszczuk, P., Rojek, K. O., Pęziński, M., Gawor, M., Pradhan, B. S., ... & Niewiadomski, P. (2020). Arhgef5 binds α-dystrobrevin 1 and regulates neuromuscular junction integrity. Frontiers in Molecular Neuroscience, 13, 104.

Fu, Y., He, P., Zhou, Y., Huang, S., Liang, L., & Liu, S. (2019). Exploring the systematic effect of N‐substituted PxxP motifs on peptoid affinity to ARHGEF5/TIM SH3 domain and its relationship with ARHGEF5/TIM activation. Proteins: Structure, Function, and Bioinformatics, 87(11), 979-991.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human ARHGEF5 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

ARHGEF5 Matched Antibody Pair (105) (CAT#: APMAB-105LY)

Antibody Pairs

ARHGEF5 Matched Antibody Pair (105) (CAT#: APMAB-105LY)

Related Products

ARHGEF5 Matched Antibody Pair (105) (CAT#: APMAB-105LY)

Mouse Anti-ARHGEF5 Recombinant Antibody (3A12-B5) (CAT#: CBMAB-A0470-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry
Request bulk or custom quote Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.