Mouse Anti-ARNT Monoclonal Antibody (CBFYH-2917) (CBMAB-H0829-FY)

Name: Mouse Anti-ARNT Monoclonal Antibody (CBFYH-2917)
SKU: CBMAB-H0829-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARNT Monoclonal Antibody (CBFYH-2917) CBMAB-H0829-FY in WB

Representative images show the Western blot analysis of AhR, ARNT, and CYP1A1 protein expressions; β-actin was significantly normalized and acts as the loading control. ...View More

Zhang, J., Zhou, B., Sun, J., Chen, H., & Yang, Z. (2021). Betulin ameliorates 7, 12-dimethylbenz (a) anthracene-induced rat mammary cancer by modulating MAPK and AhR/Nrf-2 signaling pathway. Journal of Biochemical and Molecular Toxicology, 35(7), e22779.

Mouse Anti-ARNT Monoclonal Antibody (CBFYH-2917) CBMAB-H0829-FY in WB

WB using homogenates of WT MEF and MEFCul4b-null cells (TCDD, 10 nM) with antibodies against ARNT and β-actin. Results are representative of n = 2 independent experiments. ...View More

Rijo, M. P., Diani-Moore, S., Yang, C., Zhou, P., & Rifkind, A. B. (2021). Roles of the ubiquitin ligase CUL4B and ADP-ribosyltransferase TiPARP in TCDD-induced nuclear export and proteasomal degradation of the transcription factor AHR. Journal of Biological Chemistry, 297(2).

Mouse Anti-ARNT Monoclonal Antibody (CBFYH-2917) CBMAB-H0829-FY in IP

Coimmunoprecipitation assays revealed that CORO1C-47aa competitively bound to ARNT inhibits the binding of TACC3 to its PAS-B ligand. ...View More

Li, F., Cai, Y., Deng, S., Yang, L., Liu, N., Chang, X., ... & Li, H. (2021). A peptide CORO1C-47aa encoded by the circular noncoding RNA circ-0000437 functions as a negative regulator in endometrium tumor angiogenesis. Journal of Biological Chemistry, 297(5).

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-2917

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 496-789

More Infomation

Target

Full Name

Aryl Hydrocarbon Receptor Nuclear Translocator

Introduction

This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

Human	405
Mouse	11863
Rat	25242
Cattle	281010
Sheep	443285

UniProt ID

Human	P27540
Mouse	P53762
Rat	P41739
Cattle	Q9BE97
Sheep	T2K1D9

Alternative Names

Aryl Hydrocarbon Receptor Nuclear Translocator; Class E Basic Helix-Loop-Helix Protein 2; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; BHLHe2; Hypoxia-Inducible Factor 1, Beta Subunit

Function

Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).

Biological Process

Cell differentiation Source: Ensembl
Embryonic placenta development Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of erythrocyte differentiation Source: BHF-UCL
Positive regulation of glycolytic process Source: BHF-UCL
Positive regulation of hormone biosynthetic process Source: BHF-UCL
Positive regulation of protein sumoylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial growth factor production Source: BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: BHF-UCL
Response to hypoxia Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome

Cellular Location

Nucleus

Nguyen, T., Zheng, M., Knapp, M., Sladojevic, N., Zhang, Q., Ai, L., ... & Wu, R. (2021). Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes Mellitus. Frontiers in Cell and Developmental Biology, 9, 1478.

Kim, S. A., Jo, S. H., Cho, J. H., Yu, M. Y., Shin, H. C., Kim, J. A., ... & Kim, J. H. (2020). Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4DCAF15 E3 Ligase. Molecules and cells, 43(11), 935.

Gunin, A. G., Golubtzova, N. N., & Kornilova, N. K. (2020). Aryl-Hydrocarbon Receptor Nuclear Translocator (ARNT) in Human Skin with Aging. Advances in Gerontology, 10(4), 388-392.

Scott, C., Stokes, R., Cha, K. M., Clouston, A., Eslam, M., Metwally, M., ... & Gunton, J. E. (2019). Myeloid cell deletion of Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) induces non-alcoholic steatohepatitis. Plos one, 14(12), e0225332.

Ji, S., Xin, H., & Su, E. J. (2019). Overexpression of the aryl hydrocarbon receptor nuclear translocator partially rescues fetoplacental angiogenesis in severe fetal growth restriction. Clinical Science, 133(12), 1353-1365.

Peng, T., Chen, X., Pan, Y., Zheng, Z., Wei, X., Xi, J., ... & Shang, Q. (2017). Transcription factor aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator is involved in regulation of the xenobiotic tolerance‐related cytochrome P450 CYP6DA2 in Aphis gossypii Glover. Insect molecular biology, 26(5), 485-495.

Scott, C. H., Cha, K. M., Ngai, J., Jiang, C., Cheng, K., Stokes, R. A., ... & Gunton, J. E. (2017). Hepatic Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) regulates metabolism in mice. Plos one, 12(11), e0186543.

Hunter, S. R., Vonk, A., Grey, A. K. M., & Riddick, D. S. (2017). Role of glucocorticoid receptor and pregnane X receptor in dexamethasone induction of rat hepatic aryl hydrocarbon receptor nuclear translocator and NADPH-cytochrome P450 oxidoreductase. Drug Metabolism and Disposition, 45(2), 118-129.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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