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Mouse Anti-ARNT Recombinant Antibody (CBYC-A789) (CBMAB-A3639-YC)

Provided herein is a Mouse monoclonal antibody against Human Aryl Hydrocarbon Receptor Nuclear Translocator. The antibody can be used for immunoassay techniques, such as ICC, IP, WB.
See all ARNT antibodies
Published Data
Mouse Anti-ARNT Recombinant Antibody (CBYC-A789) CBMAB-A3639-YC in WB
Western blot analysis was performed to confirm the effectiveness of siRNA treatments directed against ARNT and AhR in terms of their protein expressions following siRNA transfection, respectively. Loading controls were verified using a probe specific to b-actin. ...View More
Sciullo, E. M., Vogel, C. F., Li, W., & Matsumura, F. (2008). Initial and extended inflammatory messages of the nongenomic signaling pathway of the TCDD-activated Ah receptor in U937 macrophages. Archives of biochemistry and biophysics, 480(2), 143-155.
Mouse Anti-ARNT Recombinant Antibody (CBYC-A789) CBMAB-A3639-YC in IP
Heterodimerization of AhR with ARNT. Protein immunoprecipitation-formation of AhR-ARNT heterodimer in LS180 cells incubated for 90 min and 18 h with vehicle (DMSO; 0.1% v/v), TCDD (10 nM), Avitriptan (100 μM) and Donitriptan (100 μM). Representative immunoblots of immuno-precipitated protein eluates and total cell lysates are shown. Experiments were performed in three consecutive cell passages. ...View More
Vyhlídalová, B., Krasulová, K., Pečinková, P., Poulíková, K., Vrzal, R., Andrysík, Z., ... & Dvorak, Z. (2020). Antimigraine drug avitriptan is a ligand and agonist of human aryl hydrocarbon receptor that induces CYP1A1 in hepatic and intestinal cells. International journal of molecular sciences, 21(8), 2799.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A789
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 520-692 mapping near the C-terminus of Arnt 1 of human origin.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aryl Hydrocarbon Receptor Nuclear Translocator
Introduction
ARNT is a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it
Entrez Gene ID
405
UniProt ID
P27540
Alternative Names
Aryl Hydrocarbon Receptor Nuclear Translocator; Class E Basic Helix-Loop-Helix Protein 2; Dioxin Receptor, Nuclear Translocator; HIF-1-Beta; HIF1-Beta; BHLHe2; Hypoxia-Inducible Factor 1, Beta Subunit;
Function
Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (By similarity).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28396409).
Biological Process
Cell differentiation Source: Ensembl
Embryonic placenta development Source: Ensembl
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of erythrocyte differentiation Source: BHF-UCL
Positive regulation of glycolytic process Source: BHF-UCL
Positive regulation of hormone biosynthetic process Source: BHF-UCL
Positive regulation of protein sumoylation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of vascular endothelial growth factor production Source: BHF-UCL
Positive regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription from RNA polymerase II promoter in response to hypoxia Source: Reactome
Regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: BHF-UCL
Response to hypoxia Source: BHF-UCL
Xenobiotic metabolic process Source: Reactome
Cellular Location
Nucleus

Nguyen, T., Zheng, M., Knapp, M., Sladojevic, N., Zhang, Q., Ai, L., ... & Wu, R. (2021). Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes Mellitus. Frontiers in Cell and Developmental Biology, 9, 1478.

Kim, S. A., Jo, S. H., Cho, J. H., Yu, M. Y., Shin, H. C., Kim, J. A., ... & Kim, J. H. (2020). Aryl Sulfonamides Induce Degradation of Aryl Hydrocarbon Receptor Nuclear Translocator through CRL4DCAF15 E3 Ligase. Molecules and cells, 43(11), 935.

Gunin, A. G., Golubtzova, N. N., & Kornilova, N. K. (2020). Aryl-Hydrocarbon Receptor Nuclear Translocator (ARNT) in Human Skin with Aging. Advances in Gerontology, 10(4), 388-392.

Scott, C., Stokes, R., Cha, K. M., Clouston, A., Eslam, M., Metwally, M., ... & Gunton, J. E. (2019). Myeloid cell deletion of Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) induces non-alcoholic steatohepatitis. Plos one, 14(12), e0225332.

Ji, S., Xin, H., & Su, E. J. (2019). Overexpression of the aryl hydrocarbon receptor nuclear translocator partially rescues fetoplacental angiogenesis in severe fetal growth restriction. Clinical Science, 133(12), 1353-1365.

Peng, T., Chen, X., Pan, Y., Zheng, Z., Wei, X., Xi, J., ... & Shang, Q. (2017). Transcription factor aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator is involved in regulation of the xenobiotic tolerance‐related cytochrome P450 CYP6DA2 in Aphis gossypii Glover. Insect molecular biology, 26(5), 485-495.

Scott, C. H., Cha, K. M., Ngai, J., Jiang, C., Cheng, K., Stokes, R. A., ... & Gunton, J. E. (2017). Hepatic Aryl hydrocarbon Receptor Nuclear Translocator (ARNT) regulates metabolism in mice. Plos one, 12(11), e0186543.

Hunter, S. R., Vonk, A., Grey, A. K. M., & Riddick, D. S. (2017). Role of glucocorticoid receptor and pregnane X receptor in dexamethasone induction of rat hepatic aryl hydrocarbon receptor nuclear translocator and NADPH-cytochrome P450 oxidoreductase. Drug Metabolism and Disposition, 45(2), 118-129.

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For research use only. Not intended for any clinical use.

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